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June 29, 2016 
Our Internet email service is fixed...again.

On June 25th we identified the problem we have been having receiving emails sent to sales at arabidopsis.com  The problem began after the second of two recent software updates to a network server.  On June 25th, as a temporary fix, we swapped the network server with a backup server which restored full functioning to our primary sales email service .  However, as soon as we can, we will correct configuration issues in the server taken offline and put it back in operation because its hardware and software are more advanced.

Fred Lehle PhD

May 23, 2016
Cat. no. WT-02 Columbia Seed Contamination Update

On January 4, 2016 LEHLE SEEDS halted its WT-02 Columbia seed sales temporarily to regenerate its Columbia wild type seed stock due the confirmed presence of a small percentage of Arabidopsis plants whose rosette leaf phenotype was dissimilar to Columbia. An investigation of previous lots in our Columbia production pedigree has now been completed and the results are published in the March 2016 issue of the The Plant Cell in the following Letter to the Editor, 

Mon-Ray Shao, Vikas Shedge, Hardik Kundariya, Fredric R. Lehle, and Sally A. Mackenzie. Ws-2 Introgression in a Proportion of Arabidopsis thaliana Col-0 Stock Seed Produces Specific Phenotypes and Highlights the Importance of Routine Genetic Verification. Plant Cell 2016 28: 603-605.

To detect and and prevent any future introgressions between different Arabidopsis ecotypes from contaminating our Columbia planting seeds in the future, LEHLE SEEDS will grow, without exception, Columbia separately in time and space from the other Arabidopsis ecotypes we produce.

In addition, LEHLE SEEDS is currently implementing a new phenotype verification step in the production of our Columbia planting seeds. The purpose of this seed verification step is to confirm that all planting seeds are derived only from parents whose phenotype has been individually verified as conforming to the Columbia ecotype. More details will be provided when they are available.

Customers with questions can direct them to sales@arabidopsis.com, or telephone 1-512-388-3945 or fax us at 1-512-388-3974.

Fred Lehle, Ph.D.

March 30, 2009
ArabiPot Update - New Organizer Insert, New Subirrigation Tray, New Cat. No. PTA-126

We have recently substantially reconfigured  the ArabiPot  cat. no. PTA-10.  We removed the deep open subirrigation tray and replaced it with two new components: 1) a new low -profile subirrigation tray of the same footprint and 2) a intermediate flat insert that organizes the 18 ArabiPots into a convenient array.  The new subirrigation tray prevents accidental overwatering by setting an upper limit on the amount of each irrigation.  The new insert keeps the ArabiPots neatly organized in an 3 x 6 array, something that was needed in the original configuration.   The insert has a perforated bottom, so irrigation added to any portion of the insert is immediately distributed equally in the lower tray.  If the growth space is available, the 3 x 6 array can be expanded with multiple trays (6 total) into the common 9 x 12 array. We designate this new configuration cat. no. PTA-126, which will consist of 126 ArabiPots, 7 organizer flat inserts and 7 subirrigation trays.  With the introduction of these new components, cat. no. PTA-10 will be retired. 

The rationale behind the need for the ArabiPot remains the same and is worth repeating here. Recent research  reminds us, that as the air-spaces or pores of a plant growth medium are reduced in average physical size, a greater proportion of medium at the bottom of the pot  becomes hypoxic and remains so longer following each subirrigation.  Water-holding capacity of a growing medium increases as its pore size decreases.  This is because water flow is reduced as the pore size becomes smaller.  

The relative low air-porosity of Arabidopsis media, composed of  perlite and peat moss with variable amounts of  vermiculite, has been rated fine (Passioura 2006).  As such, Arabidopsis roots, growing in the bottom 50-70 mm of medium, regardless of pot depth, are repeatedly subjected to severely reduced oxygen levels following routine subirrigation (Passioura 2006).  While it is common to grow Arabidopsis in containers as short as 2.25" (57 mm) in height, subirrigation under such conditions requires close monitoring to avoid prolonged root water-logging which quickly slows root tissue and can stunt whole Arabidopsis plant growth.

Fortunately, there is a simple solution to avoid water-logging and hypoxia in Arabidopsis roots and that is to grow Arabidopsis plants in a deeper pot and one holding as much medium volume as possible. This is standard horticultural practice for many potted plants (Pasian 2007). 

In a deeper pot, a greater percentage of the medium near the surface will always have more pores filled with air than those in the bottom of the pot.  This provides a top region where roots can grow without the danger of hypoxia.  Given the fine porosity of common Arabidopsis media, recent  research (Passioura 2006) suggests that a pot would need to be at least 120 mm deep to guard against the possibility of ever accidentally inducing complete hypoxic conditions throughout all of a pot's growth medium.

Though counter-intuitive, square pots of the same footprint contain significantly more volume than round pots.  More medium volume increases the number of pores which improves root aeration.    Thus, given the fine air-porosity rating of Arabidopsis media, a taller square pot is much better for growth than a short round pot.  The ArabiPot is a 3.5" x 3.5" (89 x 89 mm) square pot which is a full 5" (127 mm) in depth. 

The ArabiPot  works with ARASYSTEM Aracons as well as our cat. no. SNS-03 ArabiSifter floral sleeves.  We recommend using the ArabiPot with either our cat. no. PM-15-13 AIS or cat. no. PM-25-13 PMP growing media for best results.  A single ArabiPot holds about 0.66 L of growing medium.  A box of PM-15-13 (or PM-25-13, 13 dry gallons each) will fill about 74 individual ArabiPots or 4 trays of ArabiPots (18 ArabiPots per tray).  Thus, you will need 2 boxes of either growing media to fill the 126 ArabiPots of cat. no. PTA-126. 
Pasian, CC (1997) The Ohio State University Fact Sheet HYG-1251-97 Physical Characteristics of Growing Mixes. OSU Horticulture and Crop Science  http://tinyurl.com/2cehv8

Passioura JB (2006) The perils of pot experiments.  Func. Plant Biol.33:1075-1079  

Order Cat. no. PTA-126
 ArabiPots, Organizer Flats & Subirrigation Trays for Arabidopsis

Quantity (box) Price/box*
1 $108.00
*Box includes 126 pots, 7 Organizer Inserts & 7 low-porfile subirrigation trays

To place an order

Call 1-800-881-3945
 (Toll-free in USA)

Fax 1-512-388-3974



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