Internet email service is fixed...again.
On June 25th we identified the problem we have been
having receiving emails sent to sales at arabidopsis.com The
problem began after the second of two recent software updates to a
network server. On June 25th, as a temporary fix, we swapped
the network server with a backup server which restored full
functioning to our primary sales email service . However, as
soon as we can, we will correct configuration issues in the server
taken offline and put it back in operation because its hardware and
software are more advanced.
Fred Lehle PhD
Cat. no. WT-02
Columbia Seed Contamination Update
On January 4, 2016 LEHLE SEEDS halted its WT-02 Columbia seed
temporarily to regenerate its Columbia wild type seed stock due the confirmed presence of a small percentage of Arabidopsis plants whose rosette leaf phenotype was dissimilar to
Columbia. An investigation of previous lots in our Columbia production pedigree has now been completed and the results are published in the March 2016 issue of the The Plant Cell in the following Letter to the Editor,
Mon-Ray Shao, Vikas Shedge, Hardik
Kundariya, Fredric R. Lehle, and Sally A. Mackenzie. Ws-2 Introgression in a Proportion of Arabidopsis thaliana Col-0 Stock Seed Produces Specific Phenotypes and Highlights the Importance of Routine Genetic Verification. Plant Cell 2016 28: 603-605.
To detect and and prevent any future introgressions between different Arabidopsis ecotypes from
Columbia planting seeds in the future, LEHLE SEEDS will grow,
without exception, Columbia separately in time and space from
the other Arabidopsis ecotypes we produce.
In addition, LEHLE SEEDS is currently implementing a new phenotype verification step in the production of our Columbia planting seeds. The purpose of this seed verification
step is to confirm that all planting seeds are derived only from
parents whose phenotype has been individually verified as conforming
to the Columbia ecotype. More details will be provided when they are available.
Customers with questions
can direct them to email@example.com,
or telephone 1-512-388-3945 or fax us at 1-512-388-3974.
Fred Lehle, Ph.D.
March 30, 2009
Update - New Organizer Insert, New Subirrigation Tray, New Cat.
recently substantially reconfigured the ArabiPot cat.
no. PTA-10. We removed the deep open subirrigation tray and
replaced it with two new components: 1) a new low -profile
subirrigation tray of the same footprint and 2) a intermediate
flat insert that organizes the 18 ArabiPots into a convenient
array. The new subirrigation tray prevents accidental
overwatering by setting an upper limit on the amount of each
irrigation. The new insert keeps the ArabiPots neatly
organized in an 3 x 6 array, something that was needed in the
original configuration. The insert has a perforated
bottom, so irrigation added to any portion of the insert is
immediately distributed equally in the lower tray. If the
growth space is available, the 3 x 6 array can be expanded with
multiple trays (6 total) into the common 9 x 12 array. We
designate this new configuration cat. no. PTA-126, which will
consist of 126 ArabiPots, 7 organizer flat inserts and 7
subirrigation trays. With the introduction of these new
components, cat. no. PTA-10 will be retired.
rationale behind the need for the ArabiPot remains the same and is
worth repeating here. Recent research reminds us, that as the air-spaces or pores of a plant growth medium
are reduced in average
physical size, a greater proportion of medium at the bottom of the
pot becomes hypoxic and remains so longer following each
subirrigation. Water-holding capacity of a growing medium
increases as its pore size decreases. This is because water
flow is reduced as the pore size becomes smaller.
The relative low air-porosity of Arabidopsis media,
composed of perlite and peat moss with variable amounts
of vermiculite, has been rated fine (Passioura
2006). As such, Arabidopsis roots, growing in the
bottom 50-70 mm of medium, regardless of pot depth, are repeatedly
subjected to severely reduced oxygen levels following routine
2006). While it is common to grow
Arabidopsis in containers as short as 2.25" (57 mm) in
height, subirrigation under such conditions requires close
monitoring to avoid prolonged root water-logging which quickly
slows root tissue and can stunt whole Arabidopsis plant growth.
Fortunately, there is a simple solution to avoid water-logging
and hypoxia in Arabidopsis roots and that is to
grow Arabidopsis plants in a deeper pot and one holding as much
medium volume as possible. This is standard horticultural
practice for many potted plants (Pasian
In a deeper pot, a greater
percentage of the medium near the surface will always have more
pores filled with air than those in the bottom of the pot. This provides a
top region where roots can grow without the danger of
hypoxia. Given the fine porosity of common
Arabidopsis media, recent research (Passioura
that a pot would need to be at least 120 mm deep to guard against
the possibility of ever accidentally inducing complete hypoxic
conditions throughout all of a pot's growth medium.
Though counter-intuitive, square pots of the same footprint
contain significantly more volume than round pots. More
medium volume increases the number of pores which improves root
aeration. Thus, given the fine air-porosity
rating of Arabidopsis media, a taller square pot is much better
for growth than a
short round pot. The ArabiPot is a 3.5" x
3.5" (89 x 89 mm) square pot which is a full 5" (127 mm)
The ArabiPot works with ARASYSTEM Aracons as well as our
cat. no. SNS-03 ArabiSifter floral sleeves.
We recommend using the ArabiPot with either our cat. no. PM-15-13
AIS or cat. no. PM-25-13 PMP growing media for best results.
A single ArabiPot holds about 0.66 L of growing medium. A
box of PM-15-13 (or PM-25-13, 13 dry gallons each) will fill about
74 individual ArabiPots or 4 trays of ArabiPots (18 ArabiPots per
tray). Thus, you will need 2 boxes of either growing
media to fill the 126 ArabiPots of cat. no. PTA-126.
CC (1997) The Ohio State University Fact Sheet HYG-1251-97
Physical Characteristics of Growing Mixes. OSU Horticulture and
Crop Science http://tinyurl.com/2cehv8
Passioura JB (2006) The perils of pot
experiments. Func. Plant Biol.33:1075-1079
ArabiPots, Organizer Flats & Subirrigation Trays for Arabidopsis
includes 126 pots, 7 Organizer Inserts & 7 low-porfile subirrigation trays
To place an order
(Toll-free in USA)