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archives of previous postings

Direct transcriptional control of the Arabidopsis immune receptor FLS2 by the ethylene-dependent transcription factors EIN3 and EIL1.
Boutrot F, Segonzac C, Chang KN, Qiao H, Ecker JR, Zipfel C, Rathjen JP.

Functional specification of Arabidopsis isopropylmalate isomerases in glucosinolate and leucine biosynthesis.
He Y, Chen B, Pang Q, Strul JM, Chen S.

The function of TONNEAU1 in moss reveals ancient mechanisms of division plane specification and cell elongation in land plants.
Spinner L, Pastuglia M, Belcram K, Pegoraro M, Goussot M, Bouchez D, Schaefer DG.

Microscale analysis of amino acids using gas chromatography-mass spectrometry after methyl chloroformate derivatization.
Chen WP, Yang XY, Hegeman AD, Gray WM, Cohen JD.

A plausible mechanism for auxin patterning along the developing root.
Mironova VV, Omelyanchuk NA, Yosiphon G, Fadeev SI, Kolchanov NA, Mjolsness E, Likhoshvai VA.

The C-terminal domain of FUSCA3 negatively regulates mRNA and protein levels and mediates sensitivity to the hormones abscisic acid and gibberellic acid in Arabidopsis.
Lu QS, Dela Paz J, Pathmanathan A, Chiu RS, Tsai AY, Gazzarrini S.

Extracellular hydrophilic carboxy-terminal domain regulates the activity of TaALMT1, the aluminum-activated malate transport protein of wheat.
Furuichi T, Sasaki T, Tsuchiya Y, Ryan PR, Delhaize E, Yamamoto Y.

The AAA-ATPase AtSKD1 contributes to vacuolar maintenance of A. thaliana.
Shahriari M, Keshavaiah C, Scheuring D, Sabovljevic A, Pimpl P, Häusler RE, Hülskamp M, Schellmann S.

Salicylic acid promotes seed germination under high salinity by modulating antioxidant activity in Arabidopsis.
Lee S, Kim SG, Park CM.

Variation and fitness costs for tolerance to different types of herbivore damage in Boechera stricta genotypes with contrasting glucosinolate structures.
Manzaneda AJ, Prasad KV, Mitchell-Olds T.

Histone dynamics and roles of histone acetyltransferases during cold-induced gene regulation in Arabidopsis.
Pavangadkar K, Thomashow MF, Triezenberg SJ.

Auxin signaling participates in the adaptative response against oxidative stress and salinity by interacting with redox metabolism in Arabidopsis.
Iglesias MJ, Terrile MC, Bartoli CG, D'Ippólito S, Casalongué CA.

A Collection of Target Mimics for Comprehensive Analysis of MicroRNA Function in Arabidopsis thaliana.
Todesco M, Rubio-Somoza I, Paz-Ares J, Weigel D.

Network Modeling Reveals Prevalent Negative Regulatory Relationships between Signaling Sectors in Arabidopsis Immune Signaling.
Sato M, Tsuda K, Wang L, Coller J, Watanabe Y, Glazebrook J, Katagiri F.

Plant mitochondria use two pathways for the biogenesis of tRNAHis.
Placido A, Sieber F, Gobert A, Gallerani R, Giegé P, Maréchal-Drouard L.

The Arabidopsis peroxisomal ABC transporter, comatose, complements the Saccharomyces cerevisiae pxa1 pxa2& [delta] mutant for metabolism of long chain fatty acids and exhibits fatty acyl-coa stimulated atpase activity.
Nyathi Y, De Marcos Lousa C, van Roermund CW, Wanders RJ, Johnson B, Baldwin SA, Theodoulou FL, Baker A.

Expression of pathogenesis related genes in response to salicylic acid, methyl jasmonate and 1-aminocyclopropane-1-carboxylic acid in Malus hupehensis (Pamp.) Rehd.
Zhang J, Du X, Wang Q, Cheng X, Lv D, Xu K, Qu S, Zhang Z.

Modified CAROTENOID CLEAVAGE DIOXYGENASE8 expression correlates with altered branching in kiwifruit (Actinidia chinensis).
Ledger SE, Janssen BJ, Karunairetnam S, Wang T, Snowden KC.

Mitogen-activated protein kinase 3 and 6 regulate Botrytis cinerea-induced ethylene production in Arabidopsis.
Han L, Li GJ, Yang KY, Mao G, Wang R, Liu Y, Zhang S.

The plastidial glucose 6-phosphate/phosphate antiporter GPT1 is essential for morphogenesis in Arabidopsis embryos.
Andriotis VM, Pike MJ, Bunnewell S, Hills MJ, Smith AM.

The Rab GTPase RabG3b functions in autophagy and contributes to tracheary element differentiation in Arabidopsis.
Kwon SI, Cho HJ, Jung JH, Yoshimoto K, Shirasu K, Park OK.

AtTPS1 mediated trehalose-6-phosphate synthesis is essential for embryogenic and vegetative growth and responsiveness to ABA in germinating seeds and stomatal guard cells.
Gómez LD, Gilday A, Feil R, Lunn JE, Graham IA.

Alternative polyadenylation of antisense RNAs and flowering time control.
Hornyik C, Duc C, Rataj K, Terzi LC, Simpson GG.

Enzymes of cysteine synthesis show extensive and conserved modifications patterns that include N(alpha)-terminal acetylation.
Wirtz M, Heeg C, Samami AA, Ruppert T, Hell R.

Cloning and molecular characterization of a glycerol-3-phosphate O-acyltransferase (GPAT) gene from Echium (Boraginaceae) involved in the biosynthesis of cutin polyesters.
Mañas-Fernández A, Li-Beisson Y, Alonso DL, García-Maroto F.

Arabidopsis thaliana PGR7 Encodes a Conserved Chloroplast Protein That Is Necessary for Efficient Photosynthetic Electron Transport.
Jung HS, Okegawa Y, Shih PM, Kellogg E, Abdel-Ghany SE, Pilon M, Sjölander K, Shikanai T, Niyogi KK.

AtNOA1 modulates nitric oxide accumulation and stomatal closure induced by salicylic acid in Arabidopsis.
Sun LR, Hao FS, Lu BS, Ma LY.

KLUH/CYP78A5 promotes organ growth without affecting the size of the early primordium.
Stransfeld L, Eriksson S, Adamski NM, Breuninger H, Lenhard M.

Unraveling ferulate role in suberin and periderm biology by reverse genetics.
Serra O, Figueras M, Franke R, Prat S, Molinas M.

Protein interactors of acyl-CoA-binding protein ACBP2 mediate cadmium tolerance in Arabidopsis.
Gao W, Li HY, Xiao S, Chye ML.

AtHSBP functions in seed development and the motif is required for subcellular localization and interaction with AtHSFs.
Hsu SF, Jinn TL.

Involvement of TBL/DUF231 proteins into cell wall biology.
Bischoff V, Selbig J, Scheible WR.

Arabidopsis Annexins AnnAt1 and AnnAt4 Interact with Each Other and Regulate Drought and Salt Stress Responses.
Huh SM, Noh EK, Kim HG, Jeon BW, Bae K, Hu HC, Kwak JM, Park OK.

The role of individual amino acids in the dimerization of CR4 and ACR4 transmembrane domains.
Stokes KD, Rao AG.

Transport barriers made of cutin, suberin and associated waxes.
Schreiber L.

Dynamic-module redundancy confers robustness to the gene regulatory network involved in hair patterning of Arabidopsis epidermis.
Benítez M, Elena Alvarez-Buylla R.

Polar Auxin Transport together with AINTEGUMENTA and REVOLUTA Coordinate Early Arabidopsis Gynoecium Development.
Nole-Wilson S, Azhakanandam S, Franks RG.

The Reaction Mechanism of Allene Oxide Synthase: Interplay of Theoretical QM/MM Calculations and Experimental Investigations.
Cho KB, Lai W, Hamberg M, Raman CS, Shaik S.

The microfilament cytoskeleton plays a vital role in salt and osmotic stress tolerance in Arabidopsis.
Wang C, Zhang L, Yuan M, Ge Y, Liu Y, Fan J, Ruan Y, Cui Z, Tong S, Zhang S.

Biochemical changes associated with in vivo RbcL fragmentation by reactive oxygen species under chilling-light conditions.
Nakano R, Ishida H, Kobayashi M, Makino A, Mae T.

Identification of a novel NPR1-like gene from Nicotiana glutinosa and its role in resistance to fungal, bacterial and viral pathogens.
Zhang Y, Shi J, Liu JY, Zhang Y, Zhang JD, Guo XQ.

Dismantling of Arabidopsis thaliana mesophyll cell chloroplasts during natural leaf senescence.
Evans IM, Rus AM, Belanger EM, Kimoto M, Brusslan JA.

Genetic Dissection of Basal Resistance to Pseudomonas syringae pv. phaseolicola in accessions of Arabidopsis.
Forsyth A, Mansfield JW, Grabov N, Sinapidou E, de Torres M, Grant M.

Sequence analysis and expression of orf224 gene associated with two types of cytoplasmic male sterility in Brassica napus L.
Liu J, Li M, Wang H, Yu L, Li D.

OsCIPK31, a CBL-interacting protein kinase is involved in germination and seedling growth under abiotic stress conditions in rice plants.
Piao HL, Xuan YH, Park SH, Je BI, Park SJ, Park SH, Kim CM, Huang J, Wang GK, Kim MJ, Kang SM, Lee IJ, Kwon TR, Kim YH, Yeo US, Yi G, Son D, Han CD.

The subtelomeric region of the Arabidopsis thaliana chromosome IIIR contains potential genes and duplicated fragments from other chromosomes.
Wang CT, Ho CH, Hseu MJ, Chen CM.

Characterization of the Scutellaria barbata glycosyltransferase gene and its promoter.
Chiou SJ, Liu WY, Fang CL, Lin TY.

Insights into lignin primary structure and deconstruction from Arabidopsis thaliana COMT (caffeic acid O-methyl transferase) mutant Atomt1.
Moinuddin SG, Jourdes M, Laskar DD, Ki C, Cardenas CL, Kim KW, Zhang D, Davin LB, Lewis NG.

A combined Zn/Cd sensor and Zn/Cd export regulator in a heavy metal pump.
Baekgaard L, Mikkelsen MD, Soerensen DM, Hegelund JN, Persson DP, Mills RF, Yang Z, Husted S, Andersen JP, Buch-Pedersen MJ, Schjoerring JK, Williams LE, Palmgren MG.

Identification of phosphomethylethanolamine N-methyltransferase from Arabidopsis and its role in choline and phospholipid metabolism.
Begora MD, Macleod MJ, McCarry BE, Summers PS, Weretilnyk EA.

Imprinting genes and it's expression in Arabidopsis.
Zhang HY, Xu PZ, Yang H, Wu XJ.

The plastid hexokinase pHXK: A node of convergence for sugar and plastid signals in Arabidopsis.
Zhang ZW, Yuan S, Xu F, Yang H, Zhang NH, Cheng J, Lin HH.

Analysis of promoter activity of members of the PECTATE LYASE-LIKE (PLL) gene family in cell separation in Arabidopsis.
Sun L, van Nocker S.

Continuous-time modeling of cell fate determination in Arabidopsis flowers.
van Mourik S, van Dijk AD, de Gee M, Immink RG, Kaufmann K, Angenent GC, van Ham RC, Molenaar J.

Transgenic poplar expressing Arabidopsis NDPK2 enhances growth as well as oxidative stress tolerance.
Kim YH, Kim MD, Choi YI, Park SC, Yun DJ, Noh EW, Lee HS, Kwak SS.

BRX promotes Arabidopsis shoot growth.
Beuchat J, Scacchi E, Tarkowska D, Ragni L, Strnad M, Hardtke CS.

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Sales Manager Messages                                                           archives
01 Jan 2010  Effective January 1, 2010, LEHLE SEEDS has instituted a new Customs Documentation Preparation (CDP) fee. The CDP fee will be a flat $75 and will be applied to all orders shipped to destinations outside the U.S. The change is necessary to correct a long-standing imbalance inherent in our "One World" product pricing policy. The imbalance arises because international orders require additional customs documentation not required of domestic orders. Hence, preparation of said customs documents incur significant internal staffing costs, which previously have not been properly accounted for.

14 Aug 2009  Effective today, a handling charge of $15 will be added to all seed orders totaling less than $25.

16 Mar 2008  Effective today, prices of ARASYSTEM components will be increased across the product line an average of 4.0% to reflect the continuing devaluation of the U.S. dollar relative to the euro.

28 Jan 2008  Effective today, the price of our cat. no. DA-10 OptiVISOR Binocular Magnifier was increased 6.1% due to an equivalent rise in the manufacturer's wholesale price.  Prices were also increased, for the same reason, in associated OptiVISER accessories including cat. no. LP-01 OptiLOUPE monocular lens (5.3%), cat. no. LT-06 Optivisor Clip-on Light Attachment (3.9%) and cat. no. LT-2204 Optivisor Clip-on Light Attachment Replacement Bulb (7.7%).

06 Nov 2007  Effective today, prices of ARASYSTEM components will be increased across the product line an average of 1.61% to reflect the continuing devaluation of the U.S. dollar relative to the euro.

24 Sep 2007  Effective today, prices of ARASYSTEM components will be increased across the product line an average of 3.41% to reflect the continuing devaluation of the U.S. dollar relative to the euro.

08 May 2007 Custom Project Price Guide - Fall 2007 

Pricing for custom project services for the Fall 2007 production cycle are shown in the following table.  

Destination of Project Products

USA

Foreign

Genome Classification

Service

Non-
Transgenic
Transgenic Non-
Transgenic
Transgenic
Custom EMS mutation for M1 seeds  $2,090 $2,290 $2,090 $2,590
Custom EMS mutation for M2 seeds¥ $2,140 $2,340 $2,140 $2,640
Custom M1 grow-out & selfing to M2, 12 flats (minimum)§ $2,900 $3,200 $2,900 $3,200
Total $5,040 $5,540 $5,040 $5,840
Fee covers mutation and M1 seed drying services.   A maximum of 1.5 grams of seeds are mutated per batch and then dried and shipped.  Includes chemical waste neutralization, treatment and disposal services.   Where applicable, includes extra fees associated with transgenic biologicals such as their 1) special handling, containment and disposal and 2) compliance with USDA APHIS regulations to obtain a project specific importation permit.  Shipping and any related costs are not included.  A $500 retainer is required to engage this service. Billing is monthly thereafter.

¥  Fee covers mutation and M1 seed stratification services.  A maximum of 1.5 grams of seeds are mutated per batch and stratified at 5 C in preparation of planting. Fees for actual planting and grow-out of M1 seeds are covered in following service.  Includes chemical waste neutralization, treatment and disposal services.  Where applicable, includes extra fees associated with transgenic biologicals such as their 1) special handling, containment and disposal and 2) compliance with any or all  USDA APHIS regulations to obtain a project specific importation permit.  Shipping and any related costs are not included.  A $500 retainer is required to engage this service. Billing is monthly thereafter.

§ Fee covers M1 seed sowing, growing, maintaining, selfing, harvesting, cleaning and packaging of M2 progeny seeds for 4-5 months.  This service is paired with and follows the Custom EMS mutation service for M2 (above).  M1 seed are planted to achieve a target stand of 1,100  M1 parents on each of a minimum of twelve 28 x 56 cm greenhouse flats.  Sowing rate is 0.06 grams per flat.  Add $242 or $267 for each additional flat for non-transgenic and transgenic seeds, respectively. Fee includes a stand count, embryo test for mutation frequency estimation and remote monitoring of project via images posted to our web site. Where applicable, includes extra fees associated with rendering harmless and disposing of transgenic biologicals or medium containing  their residues.  Shipping and any related costs are not included.  Billing is monthly.  

19 Feb 2007  Effective today, prices of ARASYSTEM components will be increased across the product line an average of 2.3% to reflect recent devaluation in the dollar.

Production Manager Messages                                                     archives
19 Apr 2007  Notice of Custom Project Schedule

The schedule for custom projects for the remainder of 2007 and the first half of 2008 have been finalized.  Custom projects requiring a grow-out of M2 seeds must be initiated either in the months of September 2007 or February 2008.  This scheduling is required so custom projects coincide with our regular growth room operations.  LEHLE SEEDS currently idles its growth rooms from June to August for insect control, equipment maintenance, cleaning and disinfection.  The time is also used for processing chemical waste and growth medium waste prior to disposal.  Important deadlines are summarized in the following two sections.

  Fall 2007 Custom Projects - Reservations now being accepted

15 Jul 2007 - Last day to submit application for importation permit for September 2007 custom projects where client is 1) outside the U.S.A. and seeds are 2) transgenic.

01 Sep 2007 - Last day to receive seeds for September 2007 custom projects.  Seeds received after this date will be scheduled for February 2008.

01 Sep 2007 - 30 Sep 2007 Dates when custom projects with M2 grow-out will be initiated.  Projects not initiated by 30 Sep 2007 will be scheduled for February 2008.

31 Jan 2008 (or earlier) - Fall 2007 custom project harvesting complete.

  Spring 2008 Custom Projects - Reservations now being accepted

15 Dec 2007 - Last day to submit application for importation permit for February 2008 custom projects where client is 1) outside the U.S.A. and seeds are 2) transgenic.

01 Feb 2008 - Last day to receive seeds for September 2007 custom projects.  Seeds received after this date will be scheduled for September 2008.

01 Feb 2008 - 29 Feb 2008 Dates when custom projects with M2 grow-out  will be initiated.  Projects not initiated by 29 Feb 2008 will be scheduled for Sep 2008.

30 Jun 2008 (or earlier) - Spring 2008 custom project harvesting complete.

        Additional information concerning custom projects

Space in our growing rooms is limited and projects are assigned to the available space on a first come, first serve basis.  Projects that involve the importation of transgenic seeds into the U.S. will require LEHLE SEEDS to obtain a special USDA APHIS importation permit.  The permit process is such that the application should be started in July 2007 for the September custom projects and in December 2007 for the February 2008 custom projects.  For September 2007 projects, seeds must be in our hands no later than September 1, 2007.  For February 2008 projects, seeds must be in our hands no later than February 1, 2008.  Transgenic as well as non-transgenic seeds can be submitted, but transgenic seeds can only be imported to the USA under a special USDA APHIS which LEHLE SEEDS will obtain for its foreign customers before importation.  Contact Fred Lehle, Ph.D. by email if you have additional questions or interest. 

            Custom projects involving no M1 grow-out

Custom projects with immediate return of air-dried M1 seeds will be conducted only during the months of September - October 2007 and February - March 2008.

 
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