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archives of previous postings

Control of endoreduplication of trichome by RPT2a, a subunit of the 19S proteasome in Arabidopsis.
Sako K, Maki Y, Imai KK, Aoyama T, Goto DB, Yamaguchi J.

Dynamic morphologies of pollen plastids visualised by vegetative-specific FtsZ1-GFP in Arabidopsis thaliana.
Fujiwara MT, Hashimoto H, Kazama Y, Hirano T, Yoshioka Y, Aoki S, Sato N, Itoh RD, Abe T.

The tissue expression pattern of the AtGRP5 regulatory region is controlled by a combination of positive and negative elements.
Mangeon A, Magioli C, Tarré E, Cardeal V, Araujo C, Falkenbach E, Rocha CA, Rangel-Lima C, Sachetto-Martins G.

Salt Stress Causes Peroxisome Proliferation, but Inducing Peroxisome Proliferation Does Not Improve NaCl Tolerance in Arabidopsis thaliana.
Mitsuya S, El-Shami M, Sparkes IA, Charlton WL, De Marcos Lousa C, Johnson B, Baker A.

SIAMESE Cooperates with the CDH1-like Protein CCS52A1 to Establish Endoreplication in Arabidopsis thaliana Trichomes.
Kasili R, Walker JD, Simmons LA, Zhou J, De Veylder L, Larkin JC.

Modelling temperature-compensated physiological rates, based on the coordination of responses to temperature of developmental processes.
Parent B, Turc O, Gibon Y, Stitt M, Tardieu F.

cpSecA, a thylakoid protein translocase subunit, is essential for photosynthetic development in Arabidopsis.
Liu D, Gong Q, Ma Y, Li P, Li J, Yang S, Yuan L, Yu Y, Pan D, Xu F, Wang NN.

A tomato strigolactone-impaired mutant displays aberrant shoot morphology and plant interactions.
Koltai H, Lekkala SP, Bhattacharya C, Mayzlish-Gati E, Resnick N, Wininger S, Dor E, Yoneyama K, Yoneyama K, Hershenhorn J, Joel DM, Kapulnik Y.

Polar localization and degradation of Arabidopsis boron transporters through distinct trafficking pathways.
Takano J, Tanaka M, Toyoda A, Miwa K, Kasai K, Fuji K, Onouchi H, Naito S, Fujiwara T.

Functional identification of two non-redundant Arabidopsis {alpha}(1,2)fucosyltransferases specific to arabinogalactan-proteins.
Wu Y, Williams M, Bernard S, Driouich A, Showalter AM, Faik A.

Genome-wide evidence for efficient positive and purifying selection in Capsella grandiflora, a plant species with a large effective population size.
Slotte T, Foxe JP, Hazzouri KM, Wright SI.

A plastid-targeted heat shock cognate 70kDa protein interacts with the Abutilon mosaic virus movement protein.
Krenz B, Windeisen V, Wege C, Jeske H, Kleinow T.

The Arabidopsis AP2/ERF transcription factor RAP2.6 participates in ABA, salt and osmotic stress responses.
Zhu Q, Zhang J, Gao X, Tong J, Xiao L, Zhang H.

ATP-dependent proteases in biogenesis and maintenance of plant mitochondria.
Janska H, Piechota J, Kwasniak M.

Maximum yields of microsomal-type membranes from small amounts of plant material without requiring ultracentrifugation.
Abas L, Luschnig C.

Expression of BvGLP-1 Encoding a Germin-Like Protein from Sugar Beet in Arabidopsis thaliana Leads to Resistance Against Phytopathogenic Fungi.
Knecht K, Seyffarth M, Desel C, Thurau T, Sherameti I, Lou B, Oelmüller R, Cai D.

Brassinosteroid Signal Transduction from Receptor Kinases to Transcription Factors.
Kim TW, Wang ZY.

Histone Methylation in Higher Plants.
Liu C, Lu F, Cui X, Cao X.

Control of Arabidopsis apical-basal embryo polarity by antagonistic transcription factors.
Smith ZR, Long JA.

Feature-incorporated alignment based ligand-binding residue prediction for carbohydrate binding modules.
Chou WY, Chou WI, Pai TW, Lin SC, Jiang TY, Tang CY, Chang MD.

Combined transcript and metabolite profiling of Arabidopsis thaliana grown under widely variant growth conditions facilitates the identification of novel metabolite mediated regulation of gene expression.
Hannah MA, Caldana C, Steinhauser D, Balbo I, Fernie AR, Willmitzer L.

Modulation of transcriptome and metabolome of tobacco by Arabidopsis transcription factor, AtMyb12, leads to insect resistance.
Misra P, Pandey A, Tiwari M, Chandrashekar K, Sidhu OP, Asif MH, Chakrabarty D, Singh PK, Trivedi PK, Nath P, Tuli R.

Differential impact of lipoxygenase 2 and jasmonates on natural and stress-induced senescence in Arabidopsis thaliana.
Seltmann MA, Stingl NE, Lautenschlaeger JK, Krischke M, Mueller MJ, Berger S.

RNA PROCESSING FACTOR2 Is Required for 5 ' End Processing of nad9 and cox3 mRNAs in Mitochondria of Arabidopsis thaliana.
Jonietz C, Forner J, Hölzle A, Thuss S, Binder S.

Arabidopsis RNA-Dependent RNA Polymerases and Dicer-Like Proteins in Antiviral Defense and Small Interfering RNA Biogenesis during Turnip Mosaic Virus Infection.
Garcia-Ruiz H, Takeda A, Chapman EJ, Sullivan CM, Fahlgren N, Brempelis KJ, Carrington JC.

PtAAP11, a high affinity amino acid transporter specifically expressed in differentiating xylem cells of poplar.
Couturier J, de Fa˙ E, Fitz M, Wipf D, Blaudez D, Chalot M.

Temporal analysis of natural variation for the rate of leaf production and its relationship with flowering initiation in Arabidopsis thaliana.
Méndez-Vigo B, Andrés MT, Ramiro M, Martínez-Zapater JM, Alonso-Blanco C.

Molecular characterization of FLOWERING LOCUS T-like genes of apple (Malus x domestica Borkh.).
Kotoda N, Hayashi H, Suzuki M, Igarashi M, Hatsuyama Y, Kidou SI, Igasaki T, Nishiguchi M, Yano K, Shimizu T, Takahashi S, Iwanami H, Moriya S, Abe K.

Cytokinin receptor antagonists derived from 6-benzylaminopurine.
Nisler J, Zatloukal M, Popa I, Doležal K, Strnad M, Spíchal L.

Plastid transport and metabolism of C(3) and C(4) plants-comparative analysis and possible biotechnological exploitation.
Weber AP, von Caemmerer S.

KLUH/CYP78A5-Dependent Growth Signaling Coordinates Floral Organ Growth in Arabidopsis.
Eriksson S, Stransfeld L, Adamski NM, Breuninger H, Lenhard M.

TobEA: an atlas of tobacco gene expression from seed to senescence.
Edwards KD, Bombarely A, Story GW, Allen F, Mueller LA, Coates SA, Jones L.

Subcellular compartmentation of glutathione in dicotyledonous plants.
Zechmann B, Müller M.

Extensive Divergence in Alternative Splicing Patterns After Gene and Genome Duplication During the Evolutionary History of Arabidopsis.
Zhang PG, Huang SZ, Pin AL, Adams KL.

Photorespiration: current status and approaches for metabolic engineering.
Maurino VG, Peterhansel C.

Coordinated regulation of anthocyanin biosynthesis in Chinese bayberry (Myrica rubra) fruit by a R2R3 MYB transcription factor.
Niu SS, Xu CJ, Zhang WS, Zhang B, Li X, Lin-Wang K, Ferguson IB, Allan AC, Chen KS.

Over-expression of mango (Mangifera indica L.) MiARF2 inhibits root and hypocotyl growth of Arabidopsis.
Wu B, Li YH, Wu JY, Chen QZ, Huang X, Chen YF, Huang XL.

Two similar but distinct second intron fragments from tobacco AGAMOUS homologs confer identical floral organ-specific expression sufficient for generating complete sterility in plants.
Yang Y, Singer SD, Liu Z.

Three highly similar formate dehydrogenase genes located in the vicinity of the B4 resistance gene cluster are differentially expressed under biotic and abiotic stresses in Phaseolus vulgaris.
David P, Colas des Francs-Small C, Sévignac M, Thareau V, Macadré C, Langin T, Geffroy V.

Arabidopsis AtSerpin1: crystal structure and in vivo interaction with its target protease responsive to desiccation-21 (RD21).
Lampl N, Budai-Hadrian O, Davydov O, Joss TV, Harrop SJ, Curmi PM, Roberts TH, Fluhr R.

Interdependence of Endomembrane Trafficking and Actin Dynamics during Polarized Growth of Arabidopsis Pollen Tubes.
Zhang Y, He J, Lee D, McCormick S.

Export of vacuolar manganese by AtNRAMP3 and AtNRAMP4 is required for optimal photosynthesis and growth under manganese deficiency.
Lanquar V, Ramos MS, Lelievre F, Barbier-Brygoo H, Krieger-Liszkay A, Kramer U, Thomine S.

Regulated Ethylene Insensitivity Through the Inducible Expression of the Arabidopsis etr1-1 Mutant Ethylene Receptor in Tomato.
Gallie DR.

Cloning of papaya chromoplast specific lycopene {beta}-cyclase, CpCYC-b, controlling fruit flesh color reveals conserved microsynteny and a recombination hotspot.
Blas AL, Ming R, Liu Z, Veatch OJ, Paull RE, Moore PH, Yu Q.

Transcriptional Profiling of the Arabidopsis Iron Deficiency Response Reveals Conserved Transition Metal Homeostasis Networks.
Yang TJ, Lin WD, Schmidt W.

Phosphate Regulation of Lipid Biosynthesis in Arabidopsis is Independent of the Mitochondrial Outer Membrane DGS1 Complex.
Moellering ER, Benning C.

AtNUDX6, an ADP-ribose/NADH pyrophosphohydrolase in Arabidopsis, positively regulates NPR1-dependent salicylic acid signaling.
Ishikawa K, Yoshimura K, Harada K, Fukusaki E, Ogawa T, Tamoi M, Shigeoka S.

Identification of shared single copy nuclear genes in Arabidopsis, Populus, Vitis and Oryza and their phylogenetic utility across various taxonomic levels.
Duarte JM, Wall PK, Edger PP, Landherr LL, Ma H, Pires JC, Leebens-Mack J, Depamphilis CW.

A rapid and robust assay for detection of S-phase cell cycle progression in plant cells and tissues by using ethynyl deoxyuridine.
Kotogány E, Dudits D, Horváth GV, Ayaydin F.

Protocol: a rapid and economical procedure for purification of plasmid or plant DNA with diverse applications in plant biology.
Li JF, Li L, Sheen J.

Arabidopsis RAB geranylgeranyl transferase beta subunit mutant is constitutively photomorphogenic and has shoot growth and gravitropic defects.
Hála M, Soukupová H, Synek L, Zárský V.

Metabolic profiling and cytological analysis of proanthocyanidins in immature seeds of flavonoid accumulation mutants of Arabidopsis thaliana.
Kitamura S, Matsuda F, Tohge T, Yonekura-Sakakibara K, Yamazaki M, Saito K, Narumi I.

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Sales Manager Messages                                                           archives
01 Jan 2010  Effective January 1, 2010, LEHLE SEEDS has instituted a new Customs Documentation Preparation (CDP) fee. The CDP fee will be a flat $75 and will be applied to all orders shipped to destinations outside the U.S. The change is necessary to correct a long-standing imbalance inherent in our "One World" product pricing policy. The imbalance arises because international orders require additional customs documentation not required of domestic orders. Hence, preparation of said customs documents incur significant internal staffing costs, which previously have not been properly accounted for.

14 Aug 2009  Effective today, a handling charge of $15 will be added to all seed orders totaling less than $25.

16 Mar 2008  Effective today, prices of ARASYSTEM components will be increased across the product line an average of 4.0% to reflect the continuing devaluation of the U.S. dollar relative to the euro.

28 Jan 2008  Effective today, the price of our cat. no. DA-10 OptiVISOR Binocular Magnifier was increased 6.1% due to an equivalent rise in the manufacturer's wholesale price.  Prices were also increased, for the same reason, in associated OptiVISER accessories including cat. no. LP-01 OptiLOUPE monocular lens (5.3%), cat. no. LT-06 Optivisor Clip-on Light Attachment (3.9%) and cat. no. LT-2204 Optivisor Clip-on Light Attachment Replacement Bulb (7.7%).

06 Nov 2007  Effective today, prices of ARASYSTEM components will be increased across the product line an average of 1.61% to reflect the continuing devaluation of the U.S. dollar relative to the euro.

24 Sep 2007  Effective today, prices of ARASYSTEM components will be increased across the product line an average of 3.41% to reflect the continuing devaluation of the U.S. dollar relative to the euro.

08 May 2007 Custom Project Price Guide - Fall 2007 

Pricing for custom project services for the Fall 2007 production cycle are shown in the following table.  

Destination of Project Products

USA

Foreign

Genome Classification

Service

Non-
Transgenic
Transgenic Non-
Transgenic
Transgenic
Custom EMS mutation for M1 seeds  $2,090 $2,290 $2,090 $2,590
Custom EMS mutation for M2 seedsĄ $2,140 $2,340 $2,140 $2,640
Custom M1 grow-out & selfing to M2, 12 flats (minimum)§ $2,900 $3,200 $2,900 $3,200
Total $5,040 $5,540 $5,040 $5,840
Fee covers mutation and M1 seed drying services.   A maximum of 1.5 grams of seeds are mutated per batch and then dried and shipped.  Includes chemical waste neutralization, treatment and disposal services.   Where applicable, includes extra fees associated with transgenic biologicals such as their 1) special handling, containment and disposal and 2) compliance with USDA APHIS regulations to obtain a project specific importation permit.  Shipping and any related costs are not included.  A $500 retainer is required to engage this service. Billing is monthly thereafter.

Ą  Fee covers mutation and M1 seed stratification services.  A maximum of 1.5 grams of seeds are mutated per batch and stratified at 5 C in preparation of planting. Fees for actual planting and grow-out of M1 seeds are covered in following service.  Includes chemical waste neutralization, treatment and disposal services.  Where applicable, includes extra fees associated with transgenic biologicals such as their 1) special handling, containment and disposal and 2) compliance with any or all  USDA APHIS regulations to obtain a project specific importation permit.  Shipping and any related costs are not included.  A $500 retainer is required to engage this service. Billing is monthly thereafter.

§ Fee covers M1 seed sowing, growing, maintaining, selfing, harvesting, cleaning and packaging of M2 progeny seeds for 4-5 months.  This service is paired with and follows the Custom EMS mutation service for M2 (above).  M1 seed are planted to achieve a target stand of 1,100  M1 parents on each of a minimum of twelve 28 x 56 cm greenhouse flats.  Sowing rate is 0.06 grams per flat.  Add $242 or $267 for each additional flat for non-transgenic and transgenic seeds, respectively. Fee includes a stand count, embryo test for mutation frequency estimation and remote monitoring of project via images posted to our web site. Where applicable, includes extra fees associated with rendering harmless and disposing of transgenic biologicals or medium containing  their residues.  Shipping and any related costs are not included.  Billing is monthly.  

19 Feb 2007  Effective today, prices of ARASYSTEM components will be increased across the product line an average of 2.3% to reflect recent devaluation in the dollar.

Production Manager Messages                                                     archives
19 Apr 2007  Notice of Custom Project Schedule

The schedule for custom projects for the remainder of 2007 and the first half of 2008 have been finalized.  Custom projects requiring a grow-out of M2 seeds must be initiated either in the months of September 2007 or February 2008.  This scheduling is required so custom projects coincide with our regular growth room operations.  LEHLE SEEDS currently idles its growth rooms from June to August for insect control, equipment maintenance, cleaning and disinfection.  The time is also used for processing chemical waste and growth medium waste prior to disposal.  Important deadlines are summarized in the following two sections.

  Fall 2007 Custom Projects - Reservations now being accepted

15 Jul 2007 - Last day to submit application for importation permit for September 2007 custom projects where client is 1) outside the U.S.A. and seeds are 2) transgenic.

01 Sep 2007 - Last day to receive seeds for September 2007 custom projects.  Seeds received after this date will be scheduled for February 2008.

01 Sep 2007 - 30 Sep 2007 Dates when custom projects with M2 grow-out will be initiated.  Projects not initiated by 30 Sep 2007 will be scheduled for February 2008.

31 Jan 2008 (or earlier) - Fall 2007 custom project harvesting complete.

  Spring 2008 Custom Projects - Reservations now being accepted

15 Dec 2007 - Last day to submit application for importation permit for February 2008 custom projects where client is 1) outside the U.S.A. and seeds are 2) transgenic.

01 Feb 2008 - Last day to receive seeds for September 2007 custom projects.  Seeds received after this date will be scheduled for September 2008.

01 Feb 2008 - 29 Feb 2008 Dates when custom projects with M2 grow-out  will be initiated.  Projects not initiated by 29 Feb 2008 will be scheduled for Sep 2008.

30 Jun 2008 (or earlier) - Spring 2008 custom project harvesting complete.

        Additional information concerning custom projects

Space in our growing rooms is limited and projects are assigned to the available space on a first come, first serve basis.  Projects that involve the importation of transgenic seeds into the U.S. will require LEHLE SEEDS to obtain a special USDA APHIS importation permit.  The permit process is such that the application should be started in July 2007 for the September custom projects and in December 2007 for the February 2008 custom projects.  For September 2007 projects, seeds must be in our hands no later than September 1, 2007.  For February 2008 projects, seeds must be in our hands no later than February 1, 2008.  Transgenic as well as non-transgenic seeds can be submitted, but transgenic seeds can only be imported to the USA under a special USDA APHIS which LEHLE SEEDS will obtain for its foreign customers before importation.  Contact Fred Lehle, Ph.D. by email if you have additional questions or interest. 

            Custom projects involving no M1 grow-out

Custom projects with immediate return of air-dried M1 seeds will be conducted only during the months of September - October 2007 and February - March 2008.

 
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