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May
11, 2007
Fall 2007
Custom Project Price Guide
The new
price guide for custom projects for the Fall of 2007 has been
set and is shown at the bottom of this page in the Sales Manager
Messages section. The
Fall and Spring scheduling of custom projects was announced
last month. This is the first major revision of custom
project pricing in over two years. It is also the first
revision ever that reflects our costs associated with compliance
with current USDA APHIS oversight concerning the containment and
disposal of Biosafety Level 1-P transgenic plant biologicals and
the importation of transgenic plant biologicals into the USA via
their Notification process and permit program. It is
also the first price revision that reflects the high costs
associated with the disposal of laboratory chemical wastes
generated by our custom projects. In addition, this price
revision for custom projects reflects recent increases in the
level of demand for these unadvertised services which are now
significantly competing with the resources of our primary
business. For these reasons, the
new Custom Project Price Guide for the Fall of 2007 contains
significant price increases over previous years. April
19, 2007
Fall 2007
Custom Project Schedule
The schedule for custom projects
for the remainder of 2007 and the first half of 2008 have been
finalized and are shown at the bottom of this page in the Production
Manager Messages section. Major changes are being made
to improve our growth room efficiency and our decision to set
aside the summer months for maintenance and energy
savings.
One major change is that custom
mutations involving M2 grow-outs will now be performed only
twice a year, i.e. in September and February. A second major
change is that custom projects requiring only M1 seeds will also
only be scheduled for four months of the year, i.e. September,
October, February and March.
Reservations for Fall 2007 projects
are now being taken. Email inquires to Fred
Lehle. Our limited space will be reserved on a first
come, first serve basis. Click here
for more details on our custom project scheduling.
April
3, 2007
New Product - ArabiSifter "Stake 'n Shake"
Innovative Combination of Two Functions - Floral Sleeve and Seed
Sieve
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Figure 1. LEHLE SEEDS
cat. no. SNS-01 ArabiSifter floral sleeve on
our square ArabiPot (see Figure 3 below) growing
container. 24-day-old Columbia Col-0 Arabidopsis is
shown growing on LEHLE SEEDS cat. no. PM-25-13 PMP Growing
Medium.
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Figure 2. Close-up edge view of
cat. no. SNS-01 ArabiSifter after being removed from mature plant
showing Arabidopsis seeds being sieved from same ArabiSifter in
which they were grown. Note how ArabiSifter retains siliques
and other plant residue while seeds easily fall through holes of
shaken ArabiSifter. In this photograph, the ArabiSifter had
only been tapped gently once or twice prior to taking the
picture so seeds could be seen both before and after
sieving. Note line of accumulated seeds still inside the
ArabiSifter. These will readily sieve through the holes
with more shaking.
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Healthy Arabidopsis plants produce lots of flowers. As a
result, flowers in nearby pots will readily entangle. One
way to prevent flower entanglement is to enclose the flowers of
each plant within a long clear plastic tube. A good
solution, but air-circulation is sometimes a problem and the tube
must be removed to thresh and clean the seeds. Harvest can be
quite tedious and time-consuming if a single hand sieve must be
cleaned completely after each plant is harvested.
LEHLE SEEDS is now offering a new innovation for flower
containment and seed harvest of our own design. We call it
the ArabiSifter and have assigned it cat. no.
SNS-01.
The ArabiSifter is simply a flared, light-weight,
transparent plastic floral sleeve that encloses an Arabidopsis
plant or plants growing in either a round or square
pot.
Similar to existing tubular enclosures, the ArabiSifter
1) provides support for your
flowers and 2) acts as a physical barrier to prevent the
flowers in one pot from entangling with
those of another (Figure 1).
The ArabiSifter, however, goes two steps further. First,
the unique design of the ArabiSifter
is that each
ArabiSifter is covered with thousands of small holes. Relative to a
rigid, solid-walled tube, we believe the holes in the Stake 'n Shake
as well as its flared top will facilitate better air and moisture circulation and better heat and ethylene
dissipation in confined spaces such as in a growth room.
Validation of this claim, however, awaits the collection of
supporting data.
Secondly, at maturity, the holes in the ArabiSifter simplify
seed cleaning and save time at seed harvest. The ArabiSifter accomplishes
this by also serving as a seed sieve as follows. The mature
plant and enclosing ArabiSifter is cut from the pot at the base,
and both open ends are stapled closed. The plant material
enclosed in the ArabiSifter is then gently shaken by hand over a
sheet of paper. The shattered seeds fall through the holes which act
to sieve the seeds from the retained plant residue (Figure
2). Since each plant has its own ArabiSifter sieve that is disposable,
you save time by not having to clean and dry a permanent testing sieve
between separate harvests. Sieving efficiency with the
ArabiSifter is greater the more gentle the shaking. More
aggressive crushing of plant residue will cause more chaff and
fines to end up in the seed fraction, just like with a metal
testing sieve. Regardless of your
method or technique, one pass through the ArabiSifter will free
your seeds from the bulk of the plant residue.
The ArabiSifter is sold in a convenient, stack of sleeves that
you tear-off one at a time. The flat dimensions are 3.75"
(9.53 cm; bottom edge) x 14" ( 35.56 cm; top edge) x
21.5" (54.61 cm; length). Simply cut the bottom edge of the
ArabiSifter to fit your existing pot. Trim the top edge to
fit the height of your lighting system. The ArabiSifter fits a
wide range of both round and square plots, from a 3" (7.6 cm)
diameter round pot to our recommended square ArabiPot (shown
in Figures 1 and 3).
Order Cat.
no. SNS-01
ArabiSifter
Floral Sleeves for Arabidopsis
| Quantity
per box |
Sale
Price/box |
| 100 |
$32.64 |
| 250 |
$66.31 |
| 500 |
$114.80 |
| 1,000 |
$196.93 |
To place an order
Call 1-800-881-3945
(Toll-free in USA) Fax 1-512-388-3974
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April 3, 2007
New Product - The ArabiPot
Your Arabidopsis Roots Will Thank You.
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Figure 3. Cat. no. PTA-10
ArabiPots and Subirrigation Tray. PTA-10 box includes 180
pots and 10 trays. Pots measure 3.5" x 3.5" x
5" or 8.9 cm x 8.9 cm x 12.7 cm.
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Recent research reminds us, that as the air-spaces or pores of a plant growth medium
are reduced in average
physical size, a greater proportion of medium at the bottom of the
pot becomes hypoxic and remains so longer following each
subirrigation. Water-holding capacity of a growing medium
increases as its pore size decreases. This is because water
flow is reduced as the pore size becomes smaller.
The relative low air-porosity of Arabidopsis media,
composed of perlite and peat moss with variable amounts
of vermiculite, has been rated fine (Passioura
2006). As such, Arabidopsis roots, growing in the
bottom 50-70 mm of medium, regardless of pot depth, are repeatedly
subjected to severely reduced oxygen levels following routine
subirrigation (Passioura
2006). While it is common to grow
Arabidopsis in containers as short as 2.25" (57 mm) in
height, subirrigation under such conditions requires close
monitoring to avoid prolonged root water-logging which quickly
slows root tissue and can stunt whole Arabidopsis plant growth.
Fortunately, there is a simple solution to avoid water-logging
and hypoxia in Arabidopsis roots and that is to
grow Arabidopsis plants in a deeper pot and one holding as much
medium volume as possible. This is standard horticultural
practice for many potted plants (Pasian
2007).
In a deeper pot, a greater
percentage of the medium near the surface will always have more
pores filled with air than those in the bottom of the pot. This provides a
top region where roots can grow without the danger of
hypoxia. Given the fine porosity of common
Arabidopsis media, recent research (Passioura
2006) suggests
that a pot would need to be at least 120 mm deep to guard against
the possibility of ever accidentally inducing complete hypoxic
conditions throughout all a pot's growth medium.
Though counter-intuitive, square pots of the same footprint
contain significantly more volume than round pots. More
medium volume increases the number of pores which improves root
aeration. Thus, given the fine air-porosity
rating of Arabidopsis media, a taller square pot is much better
for growth than a
short round pot.
We are pleased to announce that we now offer such a pot, one
both tall and square, which
we call the ArabiPot (Figure 3).. The ArabiPot is a 3.5" x
3.5" (89 x 89 mm) square pot which is a full 5" (127 mm)
in depth. We offer the ArabiPot combined with subirrigation
trays as cat. no. PTA-10 ArabiPots and Trays. The PTA-10 box
includes 180 ArabiPots and 10 subirrigation trays.
The ArabiPot works with ARASYSTEM Aracons as well as our
cat. no. SNS-01 ArabiSifter floral sleeves.
We recommend using the ArabiPot with either our cat. no. PM-15-13
AIS or cat. no. PM-25-13 PMP growing media for best results.
-------------
Pasian,
CC (1997) The Ohio State University Fact Sheet HYG-1251-97
Physical Characteristics of Growing Mixes. OSU Horticulture and
Crop Science http://tinyurl.com/2cehv8
Passioura JB (2006) The perils of pot
experiments. Func. Plant Biol.33:1075-1079
Order Cat.
no. PTA-10
ArabiPot & Trays for Arabidopsis
| Quantity
(box) |
Price/box* |
| 1 |
$67.91 |
| *Box
includes 180 pots and 10 subirrigation trays |
To place an order
Call 1-800-881-3945
(Toll-free in USA) Fax 1-512-388-3974
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arabidopsis.com
STAFF |
Publisher
& Editor
Fredric R. Lehle
Art & Production
Naomi Hamel
respice, adspice, prospice
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| Sales
Manager Messages
archives |
| 08
May 2007 Custom Project Price Guide - Fall 2007
Pricing for custom project services for the
Fall 2007 production cycle are shown in the following table.
|
Destination of Project Products |
|
USA |
Foreign |
| Genome Classification |
|
Service
|
Non-
Transgenic |
Transgenic |
Non-
Transgenic |
Transgenic |
| Custom EMS mutation for M1 seeds¶ |
$2,090 |
$2,290 |
$2,090 |
$2,590 |
|
| Custom EMS mutation for M2 seeds¥ |
$2,140 |
$2,340 |
$2,140 |
$2,640 |
| Custom M1 grow-out & selfing to M2, 12 flats
(minimum)§ |
$2,900 |
$3,200 |
$2,900 |
$3,200 |
| Total |
$5,040 |
$5,540 |
$5,040 |
$5,840 |
| ¶
Fee covers mutation and M1 seed
drying services. A maximum of 1.5 grams of seeds are
mutated per batch and then dried and shipped. Includes
chemical waste neutralization, treatment and disposal
services. Where applicable, includes extra fees
associated with transgenic biologicals such as their 1) special
handling, containment and disposal and 2) compliance with USDA APHIS
regulations to obtain a project specific importation permit.
Shipping and any related costs are not included. A $500
retainer is required to engage this service. Billing is monthly
thereafter.
¥ Fee
covers mutation and M1 seed stratification services. A maximum
of 1.5 grams of seeds are mutated per batch and stratified at 5 C in
preparation of planting. Fees for actual planting and grow-out
of M1 seeds are covered in following service. Includes
chemical waste neutralization, treatment and disposal
services. Where applicable, includes
extra fees associated with transgenic biologicals such as their 1)
special handling, containment and disposal and 2) compliance with
any or all USDA APHIS regulations to obtain a project specific
importation permit. Shipping and any related costs are not
included. A $500 retainer is required to engage this service.
Billing is monthly thereafter.
§ Fee
covers M1 seed sowing, growing, maintaining, selfing, harvesting,
cleaning and packaging of M2 progeny seeds for 4-5 months.
This service is paired with and follows the Custom EMS mutation
service for M2 (above). M1
seed are planted to achieve a target stand of 1,100 M1 parents
on each of a minimum of twelve 28 x 56 cm greenhouse flats.
Sowing rate is 0.06 grams per flat. Add $242 or $267 for each
additional flat for non-transgenic and transgenic seeds,
respectively. Fee includes a stand count, embryo test for
mutation frequency estimation and remote monitoring of project via
images posted to our web site. Where applicable, includes extra fees associated
with rendering harmless and disposing of transgenic biologicals or
medium containing their residues. Shipping
and any related costs are not included. Billing is
monthly. |
19
Feb 2007 Effective today, prices of ARASYSTEM components
will be increased across the product line an average of 2.3% to reflect
recent devaluation
in the dollar.
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| Production
Manager Messages
archives |
| 19 Apr
2007 Notice of Custom Project Schedule
The schedule for custom projects for the
remainder of 2007 and the first half of 2008 have been finalized.
Custom projects requiring a grow-out of M2 seeds must be initiated either
in the months of September 2007 or February 2008. This scheduling is
required so custom projects coincide with our regular growth room
operations. LEHLE SEEDS currently idles its growth rooms from June
to August for insect control, equipment maintenance, cleaning and
disinfection. The time is also used for processing chemical waste
and growth medium waste prior to disposal. Important deadlines are
summarized in the following two sections.
Fall 2007
Custom Projects - Reservations now being accepted
15 Jul 2007 -
Last day to submit application for importation permit for September 2007
custom projects where client is 1) outside the U.S.A. and seeds are 2)
transgenic.
01 Sep 2007 -
Last day to receive seeds for September 2007 custom projects. Seeds
received after this date will be scheduled for February 2008.
01 Sep 2007 - 30 Sep
2007 Dates when custom projects with M2 grow-out will be initiated. Projects
not initiated by 30 Sep 2007 will be scheduled for February 2008.
31 Jan 2008 (or
earlier) - Fall 2007 custom project harvesting complete.
Spring 2008
Custom Projects - Reservations now being accepted
15 Dec 2007 -
Last day to submit application for importation permit for February 2008
custom projects where client is 1) outside the U.S.A. and seeds are 2)
transgenic.
01 Feb 2008 -
Last day to receive seeds for September 2007 custom projects. Seeds
received after this date will be scheduled for September 2008.
01 Feb 2008 - 29 Feb
2008 Dates when custom projects with M2 grow-out will be initiated. Projects
not initiated by 29 Feb 2008 will be scheduled for Sep 2008.
30 Jun 2008 (or
earlier) - Spring 2008 custom project harvesting complete.
Additional information concerning custom projects
Space in our growing rooms is limited and
projects are assigned to the available space on a first come, first serve
basis. Projects that involve the importation of transgenic seeds
into the U.S. will require LEHLE SEEDS to obtain a special USDA APHIS
importation permit. The permit process is such that the application
should be started in July 2007 for the September custom projects and in
December 2007 for the February 2008 custom projects. For
September 2007 projects, seeds must be in our hands no later than
September 1, 2007. For February 2008 projects, seeds must be in our
hands no later than February 1, 2008. Transgenic as well as
non-transgenic seeds can be submitted, but transgenic seeds can only be
imported to the USA under a special USDA APHIS which LEHLE SEEDS will
obtain for its foreign customers before importation. Contact Fred
Lehle, Ph.D. by email if you have additional questions or
interest.
Custom projects involving no M1 grow-out
Custom projects with immediate return of
air-dried M1 seeds will be conducted only during the months of September -
October 2007 and February - March 2008.
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