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Title: Plant Tissue Culture Concepts and Laboratory Exercises
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Chapter Table of Contents
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PART I. HISTORY OF PLANT TISSUE CULTURE
1 Topic: History of plant tissue and cell culture - James D. Capometti, Dennis J. Gray and Robert N. Trigiano 3

PART II. SUPPORTING METHODOLOGIES
2 Topic: General laboratory requirements, media, and sterilization methods - Ellen G. Sutter 11
3 Laboratory:  Nutrition of the callus cultures - James D. Caponetti 27
4 Topic: Historical techniques - Robert N Trigiano, Kathleen R. Malueg and Effin T. Graham 33
5 Topic: Photographic methods for plant cell and tissue culture - Dennis J. Gray 41
6 Topic: Statistical analysis of plant tissue culture data - Michael E. Compton 47
PART III. PROPAGATION TECHNIQUES
7 Topic: Propagation from preexisting meristems - Michael E. Kane 61
8 Laboratory:  Micropropagation of Sygonium by shoot culture - Michael E. Kane 73
9 Laboratory:  Micropropagation of potato by node culture and microtuber production - Michael E. Kane 81
10 Laboratory:  Micropropagation of lilacs - Deborah D. McCown and Andrew J. Dawn 87
11 Topic: Propagation from nonmeristematic tissues -- Organogenesis - Otto J. Schwarz and Robert M. Beaty 95
12 Laboratory:  Direct shoot organogenesis from leaf explants of   chrysanthemum - Robert N. Trigiano and Roger A. May 105
13 Laboratory:  Shoot organogenesis from leaf explants of watermelon - Michael E. Compton and Dennis J. Gray 113
14 Laboratory:  Direct and indirect shoot organogenisis from leaves of Torenia fournieri - Mark P. Bridgen 121
15 Laboratory:  Shoot organogenesis from petunia leaves - John E. Preece 127
16 Topic: Nonzygotic embryogenesis - Dennis J. Gray 133
17 Laboratory:  Embryogenic callus and suspension cultures from  leaves of orchardgrass - Dennis J. Gray, Robert N. Trigiano and Bob V. Conger 149
18 Laboratory:  Somatic embryogenesis from seeds of melon - Dennis J. Gray 161
19 Laboratory: Somatic embryogenesis from seeds of melon - Hazel Y. Wetzerstein and Charleen Baker 167
20 Laboratory:  Direct somatic embryogenesis from leaves and flower  receptacles of cineraria - Robert N. Trigiano, Mary Catherine Scott and Kathleen R. Malueg 175
21 Laboratory:  Somatic embryogenesis from immature seeds of  yellow poplar - Scott A. Merkle  183
22 Laboratory:  Introduction of somatic embryogenesis in conifers - Stephen M. Attree, Patricia J. Rennie and Larry C. Fowke 191
PART IV. CROP IMPROVEMENT TECHNIQUES
23 Topic: Use of protoplasts for plant improvement - Michael E. Compton, James A. Saunders and Richard E. Veilleux 201
24 Laboratory:  Isolation, culture and fusion of tabacco and potato protoplasts - Richard E. Veilleux and Michael Compton 215
25 Laboratory:  Isolation of protoplasts from leaves of chrysanthemum and orchardgrass - Robert Trigiano 227
26 Laboratory: Isolation and culture of protoplasts from embryogenic yellow poplar - Scott A. Merkle 235
27 Topic: Haploid cultures - Sandra M. Reed 241
28 Laboratory:  Production of haploid tobacco plants using another culture - Sandra M. Reed 247
29 Topic:  Transformation -Melina López-Meyer, Ignacio E. Maldonado-Mendoza and Craig L. Nessler 253
30 Laboratory: Transformation of tobacco and carrot using Agrobacterium tumefaciens and expression of the ß-glucuronidase (GUS) reporter gene - Ignacio E. Maldonado, Melina López-Meyer, and Craig L. Nessler 261
31 Laboratory: Transformation of chrysanthemum leaf explants using Agrobacterium tumefaciens - Robert N. Trigiano 275
32 Laboratory: Construction and use of a simple gene gun for particle bombardment - Dennis J. Gray, Ernest Hiebert, Chia-Min Lin, Michael E. Compton and Victor P. Gaba 283
33 Topic: Germplasm preservation - Leigh E. Towill 291
34 Laboratory:  Vitrication as a method to cryopreserve shoot tips - Leigh E. Towill 297
35 Topic: Secondary product expression in vitro - Mary Ann L. Smith 305
36 Laboratory:  Pigment production in Ajuga cell cultures - Mary Ann L. Smith 311
37 Topic: In vitro plant pathology - C. Jacyn Baker, Norton M. Mock and Elizabeth W. Orlandi 319
PART V. SPECIAL APPLICATIONS
38 Topic: Commercial laboratory production - Gayle R. L. Suttle 331
39 Topic: Indexing for Plant pathogens - Michael J. Klopmeyer 341
PART VI. APPENDIX I. LIST OF SUPPLIERS 353
PART VII. INDEX 357
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