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Title: Arabidopsis: A Laboratory Manual

Arabidopsis: A Laboratory Manual

Detlef Weigel and Jane Glazebrook (2002)

Cold Spring Harbor Laboratory Press

ISBN: 0-87969-573-0
(hard cover with wire spiral bind)

12 chapter,  354 pages

Publisher's summary (on back cover)
Arabidopsis thaliana has emerged as the model organism of choice for a wide rage of basic and applied research into plant biology. This is largely due to the strength of Arabidopsis for genetic approaches, and, with the completion of its genome sequence, for genomic approaches as well. Our intention was to provide a simple guide to the application of Arabidopsis genetics to studies of plant biology. We hope that this volume will be a useful laboratory manual for those new to Arabidopsis  genetics, as well as a reference for more established investigators when they require a commonly used protocol with which they are not familiar.

We have organized the manual in the order that a typical project proceeds. The early chapters cover growing Arabidopsis, identification of mutants, and basic genetic analysis. Later chapters cover phenotypic analyses, isolation of genes identified by mutations, and methods for studying gene expression. Appendix 1 provides Internet resources for the Arabidopsis community. We have concentrated on methods that are widely used in the community, in an attempt to provided a useful, yet affordable reference. We have not provided protocols for a large number of basic molecular biology methods that are not specific to Arabidopsis research. Readers, who are not familiar with such methods should consult one of the many excellent molecular biology manuals.

The idea of writing this manual grew out of our experiences teaching the Arabidopsis summer course at Cold Spring Harbor Laboratory. We thought that the manual for that course would be useful for the wider community, but should be expanded to cover a broader range of methods. Daphne Preuss was instrumental in the early stages of planning and compiling the manual, and she contributed one of the chapters as well as several protocols. Other protocols were provided by us, members of our laboratories, or colleagues. We are very grateful to all contributors, without whom this manual could not have been produced. They are credited at the beginning of each protocol. Many of these protocols have been widely used for a long time, and we certainly do not pretend to have invented them, we merely wrote them down.

We are indebted to the excellent staff of Cold Spring Harbor Press. Mark Curtis drew the figures, Siân Curtis did a terrific job as developmental editor; making our text suitable for publication, and production editor Dorothy Brown made it into a book. Finally, without the persistence, patience, and organization of Kaaren Janssen and Mary Cozza, this project would never hve been completed.

We hope that investigators of Arabidopsis will find this manual useful, and we welcome any feedback on the improvement of the protocols or any mistakes in them.

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