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Title: Methods in Plant Molecular Biology: A Laboratory Course Manual

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Section Table of Contents Page
 

1

Polyethylene Glycol-mediated Transfection of Tobacco LeafMesophyll Protoplasts: An Experiment in the Study of Cre-lox Recombination (M.K. Morgan, D.W. Ow) 1
2 Functional Analysis of a Plant Transcription Factor Using Transient Expression in Maize Protoplasts (L. Rosenkrans et al.) 19
3 Biolistic Transformation of Tobacco Cells with Nuclear Drug Resistance Genes (P. Maliga) 37
4 Generation of Transgenic Tobacco Plants by Cocultivation of Leaf Disks with Agrobacterium pPZP Binary Vectors (Z. Svab et al.) 55
5 Tissue Printing to Detect Proteins and RNA in Plant Tissues (J.E. Varner, Z.-H. Ye) 79
6 Immunolocalization of Proteins in Fixed and Embedded Plant Tissues (D.C. Dixon, D.F. Klessig) 95
7 In Situ Hybridization for the Detection of RNA in Plant Tissues (D.C. Dixon et al.) 111
8 In Vitro Import of Proteins into Chloroplasts (G.K. Lamppa) 141
9 Microinjection and the Study of Tissue Patterning in Plant Apices (C. van der Schoot, W.J. Lucas) 173
10 Chloroplast Run-on Transcription: Determination of the Transcriptional Activity of Chloroplast Genes (X.-W. Deng, W. Gruissem) 191
11 In Vitro Processing of Chloroplast RNA and Analysis of RNA-Protein Interactions by Ultraviolet Cross-linking(G. Schuster, W. Gruissem) 209
12 Identification of Promoter Sequences That Interact with DNA-binding Proteins (T. Manzara, W. Gruissem) 233
13 Electrophoretic Mobility-shift Assay to Characterize Pro-tein/DNA-binding Sites (U. Schindler, A.R. Cashmore) 261
14 Characterization of Protein/DNA-binding Sites Using the Methylation Interference Assay (U. Schindler, A.R. Cashmore) 279
15 Isolation of cDNAs Encoding Sequence-specific DNA-binding Proteins Using In Situ Screening (U. Schindler, A.R. Cashmore) 301
16 Phosphorylation Studies of DNA-binding Proteins (L.J. Klimczak, A.R. Cashmore) 325
17 In Vivo Genomic Footprinting by Ligation-mediated Polymerase Chain Reaction: Comparison of the Tomato rbcS-3B Promoter in Dark- and Light-grown Seedlings (P. Carrasco, W. Gruissem) 349
18 Pulsed-field Gel Electrophoresis and Yeast Artificial Chromosome Analysis of the Arabidopsis Genome (C.J. Bell et al.) 379

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