| Section |
Table of
Contents |
Page |
| 1 |
Polyethylene Glycol-mediated
Transfection of Tobacco LeafMesophyll Protoplasts: An Experiment in the Study of Cre-lox
Recombination (M.K. Morgan, D.W. Ow) |
1 |
| 2 |
Functional Analysis of a Plant
Transcription Factor Using Transient Expression in Maize Protoplasts (L. Rosenkrans et
al.) |
19 |
| 3 |
Biolistic Transformation of
Tobacco Cells with Nuclear Drug Resistance Genes (P. Maliga) |
37 |
| 4 |
Generation of Transgenic Tobacco
Plants by Cocultivation of Leaf Disks with Agrobacterium pPZP Binary Vectors (Z. Svab et
al.) |
55 |
| 5 |
Tissue Printing to Detect
Proteins and RNA in Plant Tissues (J.E. Varner, Z.-H. Ye) |
79 |
| 6 |
Immunolocalization of Proteins in
Fixed and Embedded Plant Tissues (D.C. Dixon, D.F. Klessig) |
95 |
| 7 |
In Situ Hybridization for the
Detection of RNA in Plant Tissues (D.C. Dixon et al.) |
111 |
| 8 |
In Vitro Import of Proteins into
Chloroplasts (G.K. Lamppa) |
141 |
| 9 |
Microinjection and the Study of
Tissue Patterning in Plant Apices (C. van der Schoot, W.J. Lucas) |
173 |
| 10 |
Chloroplast Run-on Transcription:
Determination of the Transcriptional Activity of Chloroplast Genes (X.-W. Deng, W.
Gruissem) |
191 |
| 11 |
In Vitro Processing of
Chloroplast RNA and Analysis of RNA-Protein Interactions by Ultraviolet Cross-linking(G.
Schuster, W. Gruissem) |
209 |
| 12 |
Identification of Promoter
Sequences That Interact with DNA-binding Proteins (T. Manzara, W. Gruissem) |
233 |
| 13 |
Electrophoretic Mobility-shift
Assay to Characterize Pro-tein/DNA-binding Sites (U. Schindler, A.R. Cashmore) |
261 |
| 14 |
Characterization of
Protein/DNA-binding Sites Using the Methylation Interference Assay (U. Schindler, A.R.
Cashmore) |
279 |
| 15 |
Isolation of cDNAs Encoding
Sequence-specific DNA-binding Proteins Using In Situ Screening (U. Schindler, A.R.
Cashmore) |
301 |
| 16 |
Phosphorylation Studies of
DNA-binding Proteins (L.J. Klimczak, A.R. Cashmore) |
325 |
| 17 |
In Vivo Genomic Footprinting by
Ligation-mediated Polymerase Chain Reaction: Comparison of the Tomato rbcS-3B Promoter in
Dark- and Light-grown Seedlings (P. Carrasco, W. Gruissem) |
349 |
| 18 |
Pulsed-field Gel Electrophoresis
and Yeast Artificial Chromosome Analysis of the Arabidopsis Genome (C.J. Bell et al.) |
379 |