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Title: Arabidopsis: A Laboratory Manual

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Table of Contents

    Chapter 4.   How to Analyze a Mutant Phenotypically, 55

  1. Growth Parameters
         Quantitative Analysis of Root Growth
         Hypocotyl Length
         Flowering Time
         Germination Rate
         Fresh Weight Gain
         Water Loss

  2. Hormone Response
         Gibberellin/Abscisic Acid/Paclobutrazol
         Auxin
         Ethylene
         Brassinosteroids

  3. Response to the Abiotic Environment
         Root Elongation Under Salt/Hormone-induced Stress
         Germination Rate
         Electrolyte Leakage
         Fresh Weight Gain
         Water Loss
         Others: Proline and Sugar Content
         Heavy Metal Stress

  4. Bacterial Pathogens
         Preparation of Bacterial Cultures and Inoculation of Plants
         Testing the Hypersensitive Response
         Assessing Bacterial Growth

    Oomycete Pathogens: Peronospora parasitica
         Reviving Frozen Stocks and Inoculating Plants
         Rating Peronospora Infections
         Preparation of Frozen Tissue Sections
         Maintaining Infections
         Diaminobenzidine Stain for Hydrogen Peroxide
         Trypan Blue Stain for Fungi, Oomycetes, and Dead Plant
        
        Cells

  5. Histology
         Preparation of Tissue Sections of Fixed Material

  6. Specialized Staining Techniques
         The PAS Reaction for Staining Cell Walls
         Alcian Blue - PAS Reaction
         Phloroglucinol Stain for Lignin
         Viral Stain for Cytoplasm
         Neutral Red Staining for Vacuoles
         Whole - mount DAPI staining and Measurement of
             DNA Content
         Nuclear Staining for Confocal Micoscopy
         Cleared Tissue for Observation of Vascular Strands
         Agarose Imprints of Surfaces

  7. Scanning Electron Microscopy
         Standard SEM Protocol
         Dental Wax Impressions to be Viewed in SEM
         SEM "Quick-and-Easy" Fixation
         Imaging of Fresh Arabidopsis Tissue in the SEM

  8. Transmission Electron Microscopy
         Standard TEM Protocol
         TEM Freeze Substitution

     References

Page

 

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