Table of Contents
Chapter 4. How to Analyze a Mutant Phenotypically, 55
Growth Parameters
Quantitative Analysis of Root Growth
Hypocotyl Length
Flowering Time
Germination Rate
Fresh Weight Gain
Water Loss
Hormone Response
Gibberellin/Abscisic Acid/Paclobutrazol
Auxin
Ethylene
Brassinosteroids
Response to the Abiotic
Environment
Root Elongation Under Salt/Hormone-induced
Stress
Germination Rate
Electrolyte Leakage
Fresh Weight Gain
Water Loss
Others: Proline and Sugar Content
Heavy Metal Stress
Bacterial Pathogens
Preparation of Bacterial Cultures and
Inoculation of Plants
Testing the Hypersensitive Response
Assessing Bacterial Growth
Oomycete Pathogens: Peronospora
parasitica
Reviving Frozen Stocks and Inoculating
Plants
Rating Peronospora Infections
Preparation of Frozen Tissue Sections
Maintaining Infections
Diaminobenzidine Stain for Hydrogen Peroxide
Trypan Blue Stain for Fungi, Oomycetes, and
Dead Plant
Cells
Histology
Preparation of Tissue Sections of Fixed
Material
Specialized Staining
Techniques
The PAS Reaction for Staining Cell Walls
Alcian Blue - PAS Reaction
Phloroglucinol Stain for Lignin
Viral Stain for Cytoplasm
Neutral Red Staining for Vacuoles
Whole - mount DAPI staining and Measurement
of
DNA Content
Nuclear Staining for Confocal Micoscopy
Cleared Tissue for Observation of Vascular
Strands
Agarose Imprints of Surfaces
Scanning Electron
Microscopy
Standard SEM Protocol
Dental Wax Impressions to be Viewed in SEM
SEM "Quick-and-Easy" Fixation
Imaging of Fresh Arabidopsis Tissue in
the SEM
Transmission Electron
Microscopy
Standard TEM Protocol
TEM Freeze Substitution
References
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