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Title: Arabidopsis: A Laboratory Manual

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Table of Contents

    Chapter 5.  How to Transform Arabidopsis, 119

  1. Vectors and Agrobacterium Hosts
         Agrobacterium strains

  2. Transformation of Agrobacterium
        
    Transformation of Agrobacterium Using Electroporation
         Transformation of Agrobacterium Using the
             Freeze-Thaw Method
         PCR Analysis of Atrobacterium 

  3. In Planta Transformation of Arabidopsis
        
    Plant Growth
         Floral Dip of Arabidopsis
         Alternative Protocol 1: Vacuum Infiltration
         Alternative Protocol 2: Spraying

  4. Selecting Transformed Plants
         Kanamycin Selection
         Basta Selection on Soil

  5. Root Transformation

     References

Page

 

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140


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