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Title: Arabidopsis: A Laboratory Manual

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Table of Contents

    Chapter 6.  How to Isolate a Gene Defined by a Mutation, 143

  1. Isolating a Gene Defined by an Insertion Mutation

  2. TAIL - PCR

  3. Isolating a Gene Known Only by the Phenotypes of Mutant
             Alleles: Positional Cloning
         STEP 1: Determining the Approximate Map Position for yfg
         STEP 2: Define the Map of yfg as Narrowly as Possible
         STEP 3: Finding YFG within the Mapped Interval
         Special Cases
         Serious Errors

  4. Purifying DNA from Arabidopsis
         CTAB DNA Miniprep
         Dellaporta DNA Miniprep
         Quick DNA Prep for PCR

     References

Page

 

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