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Title: Arabidopsis: A Laboratory Manual

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Table of Contents

    Chapter 7.  How to Study Gene Expression, 171

  1. RNA Expression
         RNA Extraction for Northern Blots and RT-PCR
         Semiquantitative Reverse Transcription followed by PCR
         In Situ Hybridization to Tissue Sections
              Radioactive In Situ Hybridization
              Nonradioactive In Situ Hybridization
         Whole-mount In Situ Hybridization

  2. Protein Expression
         Extraction of Total Protein for Western Blots
         Organelle Preparations
         FPLC Gel Filtration
         Nondenaturing Gel Electrophoresis of Proteins
         Protein Coimmunoprecipitation
         In Situ Localization of Proteins

  3. Reporter genes
         Whole-mount GUS Staining 
         Quantitative GUS Activity Assays
         Subcellular Localization of GUS- and GFP-Tagged
              Proteins in Onion Epidermal Cells
         Live-cell Imaging of GFP
         Liquid LUC Activity Assays
         LUC Imaging of Whole Plants

     References

Page

 

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212

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276


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