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Title: Methods in Molecular Biology Volume 82
ARABIDOPSIS PROTOCOLS

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Table of Contents
Page
  • Part I. Arabidopsis Culture

1. Growth of Plants and Preservation of Seeds - Randy Scholl, Luz Rivero-Lepinckas and Deborah Crist

1

2. Sterile Techniques in Arabidopsis - Peter McCourt and Kallie Keith

13

3. Control of Pests and Diseases of Arabidopsis - Mary Anderson

19

4. Establishment and Maintenance of Cell Suspension Cultures - Jaideep Mature and Csaba Koncz

27

5. Callus Culture and Regeneration - Jaideep Matur and Csaba Koncz

31

6. Protoplast Isolation, Culture, and Regeneration - Jaideep Mathur and Csaba Koncz

35
  • Part II. Purification of Subcellular Organelles and Macromolecules

7. Preparation of Physiologically Active Chloroplasts from Arabidopsis - Ljerka Kunst

43

8. Purificaiton of Mitochondria from Arabidopsis - Mathieu Klein, Stefan Binder, and Axel Brennicke

49

9. Preparation of DNA from Arabidopsis - Jianming Li and Joanne Chory

55

10. High Molecular Weight DNA Extraction from Arabidopsis - David Bouchez and Christine Camilleri

61

11. Chloroplast DNA Isolation - George S. Mourad

71

12. Purification of Mitochondrial DNA from Green Tissues of Arabidopsis - Mathieu Klein, Rudolf Hiesel, Charles André, and Axel Brennicke

79

13. Preparation of RNA - Clifford D. Carpenter and Anne E. Simon

85
  • Part III. Mutagenesis and Genetic Analysis

14. Seed Mutagenesis of Arabidopsis - Jonathan Lightner and Timothy Caspar

91

15. Genetic Analysis - Maarten Koornneef, Carlos Alonso-Blanco, and Piet Stam

105

16. Cytogenetic Analysis of Arabidopsis - John S. Heslop-Harrison

119

17. PCR-Based Identification of T-DNA Insertion Mutants - Rodney G. Winkler and Kenneth A. Feldmann

129
  • Part IV. Gene Mapping In Arabidopsis

18. The Use of Recombinant Inbred Lines (RILs) for Genetic Mapping - Carlos Alonso-Blanco, Maarten Koornneef and Piet Stam

137

19. AFLPTM Fingerprinting of Arabidopsis - Pieter Vos

147

20. Building a High-Density Genetic Map Using the AFLPTM Technology - Martin T.R. Kuiper

157

21. Use of Cleaved Amplified Polymorphic Sequences (CAPS) as Genetic Markers in Arabidopsis thaliana - Jane Glazebrook, Eliana Drenkard, Daphne Preuss, and Frederick Ausubel

173

22. Mapping Mutations with ARMS - Anton R. Schäffner

183

23. Mapping Cloned Sequences on YACs - Francis D. Agyare, Gus Lagos, Deval Lashkari, ronald W. Davis and Bertrand Lemieux

199
  • Part V. Transient and Stable Transformation

24. Transient Gene Expression in Protoplasts of Arabidopsis thaliana - Steffen Abel and Athanasios Theologis

209

25. Transient Expression of Foreign Genes in Tissues of Arabidopsis thaliana by Bombardment-Mediated Transformation - Motoaki Seki, Asako Iida and Hiromichi Morikawa

219

26. Root Transformation by Agrobacterium tumefaciens - Annette C. Vergunst, Ellen C. de Waal and Paul J.J. Hooykaas

227

27. Transformation of Arabidopsis thaliana C24 Leaf Discs by Agrobacterium tumefaciens - Eric van der Graaf and Paul J.J. Hooykas

245

28. In Planta Agrobacterium-Mediated Transformation of Adult Arabidopsis thaliana Plants by Vacuum Infiltration - Nicole Bechtold and Georges Pelletier

259

29. PEG-Mediated Protoplast Transformation with Naked DNA - Jaideep Matur and Csaba Koncz

267
  • Part VI. Gene Cloning Strategies

30. Cloning Genes of Arabidopsis thaliana by Chromosome Walking - Jeffrey Leung and Jérôme Giraudat

277

31. Chromosome Landing Using an AFLPTM -Based Strategy - Ann Van Gysel, Gerda Cnops, Peter Breyne, Marc Van Montagu, and Maria Teresa Cervera

305

32. Transposon Tagging with Ac/Ds in Arabidopsis - Deborah Long and George Coupland

315

33. Transposon Tagging with the EN-I System - Andy Periera and Mark G. M. Aarts

329

34. Cloning Genes from T-DNA Tagged Mutants - Brian P. Dilkes and Kenneth A. Feldmann

339
  • Part VII. Gene Expression Analysis

35. In Situ Hybridization - Gary N. Drews

353

36. Whole-Mount In Situ Hybridization in Plants - Janice de Almeida Engler, Marc Van Montagu and Gilbert Engler

373

37. Bacterial and Coelenterate Luciferases as Reporter Genes in Plant Cells - William H.R. Langridge and Aladar A. Szalay

385

38. ß-Glucoronidase Enzymen Histochemistry on Semithin Sections of Plastic-Embedded Arabidopsis Explants - Marc De Block and Mieke Van Lijsebettens

397

39. In Situ Hybridization to RNA in Whole Arabidopsis Plants - Qingzhong Kong and Anne E. Simon

409

40. In Vivo Footprinting in Arabidopsis - Anna-Lisa Paul and Robert J. Ferl

417

Index

431
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