|
Table of Contents |
Page |
| I |
PREFACE |
xx |
| II |
CONTRIBUTORS |
xx |
| III |
CHAPTERS: |
|
| 1. |
Growth and Storage of Agrobacterium,
Fraukje M. van Asma |
1 |
| 2. |
Simple Cultural Tests for
Identification of Agrabacterium Biovars, Hacene Bouzar, Jeffrey B.
Jones, and Andrew L. Bishopi |
9 |
| 3. |
Agrabacterium
Virulence, Jill S. Gartland |
15 |
| 4. |
Agrobacterium
tumefaciens, Chemotaxis Protocols, Charles Shaw |
29 |
| 5. |
Effect of Acetosyringone on
Growth and Oncongenic Potential of Agrobacterium tumefaciens, Patrice Dion,
Christian Belanger, Dong Xu, and Mahmood Mohammadi |
37 |
| 6. |
Binary Ti Plasmid Vectors, Gynheung
An |
47 |
| 7. |
Leaf Disk
Transformation, Ian S. Curtis, Michael R. Davey, and J. Brian Power |
59 |
| 8. |
Agrobacterium-Mediated
Tranformation of Protoplasts from Oilseed Rape (Brassica napus L.), Jurgen E.
Thomzik |
71 |
| 9. |
Agrobacterium-Mediated
Transformation of Stem Disks from Oilseed Rape (Brassica napus L.), Jurgen
E. Thomzik |
79 |
| 10. |
Peanut Transformation, Elisabeth
Mansur, Cristiano Lacorte, and William R. Krul |
87 |
| 11. |
Transformation of Soybean
(Glycine max) via Agrobacterium tumefaciens and Analysis of Transformed Plants, Pauls
P. Chee and Jerry L. Slightom |
101 |
| 12. |
Agrobacterium-Mediated
Transformation of Potato Genotypes, Amar Kumar |
121 |
| 13. |
Agrobacterium-Mediated
Transformation of Soft Fruit Rubus ( Rubus, Ribes,and Fragaria) Julie
Graham, Ronnie J. McNicol, and Amar Kumar |
129 |
| 14. |
High Frequency and Efficient
Agrobacterium-Mediated Transformation of Arabidopsis thalania Ecotypes "C24" and
"Landsberg erecta" Using bacterium tumefaciens, Mehdi Barghchi |
135 |
| 15. |
Transformation Protocols for
Broadleaved Trees, Trevor M. Fenning and Kevin M. A. Gartland |
149 |
| 16. |
Agrabacterium-Mediated
Antibiotic Resistance for Selection of Somatic Hybrids: The Genus Lycopersicon as a
Model System, Michael R. Davey, Rajendra S. Patil, Kenneth C. Lowe, and Lohn
B. Power |
167 |
| 17. |
Histochemical GUS Analysis, Stanislav
Vitha, Karel Baneš, Julian P. Phillips, and Kevin M. A. Gartland |
185 |
| 18. |
Fluorometric GUS Analysis
for Transformed Plant Material, Kevan Gartland, Julia P. Phillips, Stanislav Vitha, and
Karel Benes |
195 |
| 19. |
The Detection of Neomycin
Phosphotransferase Activity in Plant Extracts, Ortun Mittelsten Scheid and
Gabriele Neuhaus-Url |
201 |
| 20. |
The Plant Oncogenes rolA,
B, C from Agrobacterium rhizogenes: Effects on Morphology, Development, and Hormone
Metabolism, Tony Michael and Angelo Spena |
207 |
| 21. |
Quantifying Polyamines in Agrobacterium
rhizogenes Strains and in Ri Plasmid Transformed Cells, Nello Bagni,
Marisa Mengoli, and Shigeru Matsuzaki |
223 |
| 22. |
IAA Analysis in Transgenic
Plants, John F. Hall, Sarah J. Brown, and Kevan M.A. Gartland |
237 |
| 23. |
Quantifying Phytohormones in
Transformed Plants, Els Prinsen, Pascale Redig, Miroslav Strnad, Ivan Galís,
Walter Van Dongen, and Henri Van Onckelen |
245 |
| 24. |
Manipulating Photostnthesis
in Transgenic Plants, Jacqueline S. Knight, Francisco Madueño, Simon A. Barnes,
and John C. Gray |
263 |
| 25. |
Gene Activation by T-DNA
Tagging, Klaus Fritze and Richard Walden |
281 |
| 26. |
Assessing Cadmium
Partitioning in Transgenic Plants, George J. Wagner |
295 |
| 27. |
Overexpression of
Chloroplastic Cu/Zn Superoxide Dismutase in Plants, Randy D. Allen |
309 |
| 28. |
Agroinfection, Nigel
Grimsley |
325 |
| 29. |
T-DNA Transfer to Maize
Plants, Wen-Hui Shen, Jesús Escudero, and Barbara Hohn |
343 |
| 30. |
Use of Cosmid Libraries in
Plant Transformations, Hong Ma |
351 |
| 31. |
Regulation of Agrobacterium
Gene Manipulation, Frank Dewhurst |
369 |
| 32. |
Transgenic Oilseed Rape:
How to Assess the Risk of Crossing to Wild Relatives, Alain Baranger,
Marie-Claire Kerlan, Anne-Marie Chèvre, Frédérique Eber, Patrick Vallée, and Michel
Renard |
393 |
| 33. |
Electroporation Protocols
for Agrobacterium, Garry D. Main, Stephen Reynolds, and Jill S. Gartland |
413 |
