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Title: Recombinant Proteins from Plants

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Section Table of Contents
Page
1 Rice Transformation by Agrobacterium Infection, Xiongying Cheng, Ravinder, K. Sardana, and Illimar Altosaar
1
2 Barley as a Producer of Heterologous Protein, Leena Mannen, Kristian, Aspegren, Anneli Ritala, Hanna Simola, and Teemu H. Teeri
11
3 Introducing and Expressing Genes in Legumes, Adrian D. Bavage, Mark P. Robbins, Leif Skøt, and K. Judith Webb
27
4 Use of the GUS Reporter Gene, Joy Wilkinson and Keith Lindsey
39
5 Expression of Recombinant Proteinase Inhibitors in Plants, Dominique Michaud and Thierry C. Vrain
49
6 Detection of Recombinant Viral Coat Protein in Transgenic Plants, Herta Steinkeliner and Irina Korschhineck
65
7 Synthesis of Recombinant Viral Human Cytokine GM-CSF in the Seeds of Transgenic Tobacco Plants, Ravinder K. Sardana, Peter R. Ganz, Anil Dudani, Eileen S. Tackaberry, Xiongying Cheng, and Illimar Altosaar 65
8 Tobamovirus Vectors for Expression of Recombinant Genes on Plants, Thomas H. Turpen and Stephen J. Reinl 89
9 Single-Chain Fv Antibodies Expressed in Plants, Udo Conrad, Ulrike Fiedler, Olga Artsaenko, and Julian Philips
103
10 Characterization and Applications of Plant-Derived Recombinant Antibodies, Rainer Fischer, Jürgen Drossard, Yu-Cai Liao, and Stefan Schillberg 129
11 Production of Recombinant Antibodies in Plant Suspension Cultures, Jürgen Drossard, Yu-Cai Liao, and Rainer Fischer 143
12 Production of Foreign Proteins in Tobacco Cell Suspension Culture, Véronique Gomord, Anne-Catherine Fitchette-Lainé Lise-Anne Denmat, Dominique Michaud, and Loïc Faye 155
13 Transient Gene Expression in Plant Protoplasts, Stefan Shillberg, Sabine Zimmermann, Dirk Prüfer, Detlef Schuman, and Rainer Fischer 165
14 Stability of Recombinant Protein in Plants, Dominique Michaud, Thierrt C. Vrain, Véronique Gomord, and Loïc Faye 177
15 Trafficking and Stability of Heterologous Protein, Jonathan A. Napier, Gaelle Richard, and Peter R. Shewry 189
16 Screening for Transgenic Lines with Stable and Suitable Accumulation Levels of a Heterologous Protein, Myriam De Neve, Helena Van Houdt, Anne-Marie Bruyns, Marc Van Montagu, and Ann Depicker 203
17 Manipulation of Photosynthetic Metabolism, Martin A. J. Parry, Steven P. Colliver, Pippa J. Madgwick, and Matthew J. Paul 229
18 Quantification of Heterologous Protein Levels in Transgenic Plants by ELISA, Anne-Marie Bruyns, Myriam De Neve, Geert De Jaeger, Chris De Wilde, Pierre Rouzé, and Ann Depicker 251
19 Analysis of N- and O- Glycosylation of Plant Proteins, Anne-Catherine Fitchette-Lainé, Lise-Anne Denmat, Patrice Lerouge, and Loïc Faye 271
20 Two-Dimensional Electrophoresis: A Tool for Protein Separation and Processing for Microsequencing, N.P. Eswara Reddy and M. Jacobs 291
Index 305

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