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Title: Molecular Biomethods Handbook
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Chpt.

Table of Contents

Page
1. Extraction of Total RNA from Tissues and Cultured Cells, Sandeep Raha, Mingfu Ling, and Frank Merante 1
2. Isolation of Total Cellular DNA from Tissues and Cultured Cells, Frank Merante, Sandeep Raha, and Mingfu Ling 9
3. Gel Electrophoresis of DNA, Duncan R. Smith 17
4. S1 Nuclease Analysis of RNA, Louis Lefebvre and Stéphane Viville 35
5. Detecting mRNA by Use of the Ribonuclease Protection Assay (RPA), Ralf Einspanier and Annette Plath 51
6. Gene Probes, Marilena Aquino de Muro 59
7. Nonradioactive Labeling of DNA, Thomas P. McCreery and Terrence R. Barrette 73
8. Southern Blot Analysis, Paolo A. Sabelli 77
9. Northern Blot Analysis, Paolo A. Sabelli 89
10. DNA Sequencing, Chris Spencer 95
11. Autoradiography and Fluorography, Bronwen Harvey 109
12. Mobility Shift Assays, Nigel J. Savery and Stephen J. W. Busby 121
13. cDNA Libraries, Ian G. Cowell 131
14. Genomic DNA Libraries, Bimal D. M. Theophilus 145
15. Lambda as a Cloning Vector, Rupert Mutzel

153
16. Plasmid-Derived Cloning Vector, Craig Winstan 165
17. M13 and Phagemid-Based Cloning Vectors, Craig Winstanley and Ralph Rapley 181
18. Retroviral Vectoors: From Laboratory Tools to Molecular Medicines, Richard G. Vile, Anne Tuszynski, and Simon Castleden 193
19. Baculovirus Vectors, Azeem Anzari and Vincent C. Emery 219
20. Gene Transfer and Expression in Tissue Culture Cells of Higher Eukaryotes, M. Alexandra Aitken, Selina Raguz, and Michael Antiniou 235
21. Plant Transformation, Andy prescott, Rob Briddon, and Wendy Harwood 251
22. Restriction Fragment-Length Ploymorphisms, Eileen K. Green 271
23. Genome Mapping, Jacqueline Boultwood 281
24. Genomic DNA Libraries, Bimal D. M. Theophilus 287
25. Lambda as a Cloning Vector, Rupert Mutzel

305
26. In Vitro Transcription, Martin J. Tymms 327
27. In Vitro Translation, Martin J. Tymms 335
28. Site-Directed Mutagenesis, John R. Adair and T. Paul Wallace 347
29. Transgenic Techniques, Roberta M. James and Paul Dickinson 347
30. DNA Profiling: Theory and Practice, Karen M. Sullivan 383
31. Radiolabeling of Peptides and Proteins, Arvind C. Patel and Stewart R. Matthewson 401
32. Protein Electrophoresis, Paul Richards 413
33. Free Zone Capillary Electrophoresis, David J. Begley 425
34. Protein Blotting: Principles and Applications, Peter R. Shewry and Roger J. Fido 435
35. Ion-Exchange Chromatography, David Sheehan and Richard Fitzgerald

445
36. Size-Exclusion Chromatography, Paul Cutler 451
37. Hydrophobic Interaction Chromatography, Paul A. O'Farrell 461
38. Affinity Chromatography, George W. Jack 469
39. Reversed-Phase HPLC, Bill Neville 479
40. Glycoprotein Analysis, Terry D. Butters 491
41. Protein Sequencing, Bryan J. Smith and John R. Chapman 503
42. Solid-Phase Peptide Synthesis, Gregg B. Fields 527
43. Protein Engineering, Sudhir Paul 547
44. Monoclonal Antibodies, Christopher Dean and Helmout Modjtahedi 567
45. Phage-Display Libraries, Julia E. Thompson and Andrew J. Williams

581
46. Enzyme-Linked Immunosorbent Assay (ELISA), John R. Crowther 595
47. Epitope Mapping: Identification of Antibody Binding Sites on Protein Antigens, Glenn E. Morris 619
48. Immunocytochemistry, Lorette C. Javois 631
49. Flow Cytometry, Robert E. Cunningham 653
50. Mass Spectrometry, John R. Chapman 669
51. The Technique of In Situ Hybridization: Principles and Applications, Desiré du Sart and K. H. Andy Choo 697
Index 721
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