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Title: Plant Cell Culture Protocols

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chpt.

Table of Contents

Page

Part I. Introduction
1. An Introduction to Plant-Cell Culture:  Pointers to Success, Robert D. Hall 1
Part II. Cell Culture and Plant Regeneration
2. Callus Initiation, Maintenance, and Shoot Induction in Rice, Nigel W. Blackhall, Joan P. Jotham, Kasimalai Azhakanandam, J. Brian Power, Kenneth C. Lowe, Edward C. Cooking, and Michael R. Davey 19
3. Callus Initiation, Maintenance, and Shoot Induction on Potato:  Monitoring of Spontaneous Genetic Variability In Vitro and In Vivo, Rosario F. Curry and Alan C. Cassells 31
4. Somatic Embryogenesis in Barley Suspension Cultures, Makoto Kihara, Hideyuki Funatsuki, Kazutoshi Ito, and Paul A. Lazzeri 43
5. Somatic Embryogenesis in Picea Suspension Cultures, Ulrika Egertsdotter 51
Specialized Techniques
6. Direct, Cyclic Somatic Embryogenesis of Cassava for Mass Production Purposes, Krit J. J. M. Raemakers, Evert Jacobsen, and Richard G. F. Visser 61
7. Immature Inflorescence Culture of Cereals: A Highly Responsive System for Regeneration and Transformation, Sonriza Rasco-Gaunt and Pilar Barcelo 71
8. Cryopreservation of Rice Tissue Cultures, Erica E. Benson and Paul T. Lynch 83
9. Noncryogenic, Long-Term Germplasm Storage, Ali Golmirzaie and Judith Toledo 95
Part III. Plant Propagation In Vitro
10. Micropropagation of Strawberry via Axillary Shoot Proliferation, Philippe Boxus 103
11. cDNA Libraries, Ian G. Cowell 115
Specialized Techniques
12. Clonal Propagation of Orchids, Brent Tisserat and Daniel Jones 127
13. In Vitro Propagation of Succulent Plants, Jill Gratton and Michael F. Fay

135

14. Micropropagation of Flower Bulbs: Lily and Narcissus, Merel M. Langens-Gerrits and Geert-Jan M. DeKlerk 141
15. Clonal Propagation of Woody Species, Indra S. Harry and Trevor A. Thorpe 149
16. Spore-Derived Axenic Cultures of Ferns as a Method of Propagation, Matthew V. Ford and Michael F. Fay 159
Part IV. Application for Plant Protoplasts
17. Protoplast Isolation, Culture, and Plant Regeneration from Passiflora, Paul Anthony, Wagner Otoni, J. Brian Power, Kenneth C. Lowe, and Michael R. Davey 169
18. Isolation, Culture, and Plant Regeneration of Suspension-Derived Protoplasts of Lolium, Marianne Folling and Annette Olesen 183
19. Protoplast Fusion for Symmetric Somatic Hybrid Production on Brassicaceae, Jan Fahleson and Kristina Glimelius 195
20. Production of Cybrids on Rapeseed (Brassica napus), Stephen Yarrow 211
Specialized Techniques
21. Microplast-Mediated Chromosome Transfer (MMCT) for the Direct Production of Monosomic Addition Lines, Kamisetti S. Ramulu, Paul Dijkhuis, Jan Blaas, Frans A. Krens, and Harrie A. Verhoeven

227

22. Guard Cell Protoplasts: Isolation, Culture, and Regeneration of Plants, Graham Boorse and Gary Tallman 243
23. In Vitro Fertilization with Isolated Single Gametes, Erhard Kranz 259
Part V. Protocols for Genomic Manipulation
24. Protocols for Anther and Microspore Culture of Barley, Alwine Jähne-Gärtner and Horst Lörz 269
25. Microspore Embryogenesis and In Vitro Pollen Maturation in Tobacco, Alisher Touraev and Erwin Heberle-Bors 281
26. Embryo Rescue Following Wide Crosses, Hari C. Sharma 293
Specialized Techniques
27. Mutagenesis and the Selection of Resistant Mutants, Philip J. Dix 309
28. The Generation of Plastid Mutants in Vitro, Philip J. Dix 319
Part IV. Protocols for the Introduction of Specific Genes
29. Agrobacterium-Mediated Transformation of Petunia Leaf Disks, Ingrid M. van der Meer 327
30. Transformation of Rice via PEG-Mediated DNA Uptake into Protoplasts, Karabi Datta and Swapan K. Datta 335
31. Transformation of Wheat via Particle Bombardment, Indra K. Vasil and Vimla Vasil 349
32. Plant Transformation via Protoplast Electroporation, George Bates 359
Specialized Techniques
33. Transformation of Maize via Tissue Electroporation, Kathleen D'Halluin, Els Bonne, Martien Bossut, and Rosita Le Page 367
34. Transformation of Maize Using Silicon Carbide Whiskers, Jim M. Dunwell 375
Part VII. Suspension Culture Initiation and the Accumulation of Metabolites
35. Directing Anthraquinone Accumulation via Manipulation of Morinda Suspension Cultures, Marc J. M. Hagendoorn, Diaane C. L. Jamar, and Linus H. W. van der Plas 383
36. Betalains: Their Accumulation and Release In Vitro, Christopher S. Hunter and Nigel J. Kilby

403

Appendix 411
Index 415
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