Table of Contents
|
Page |
| Preface
|
iii |
| Acknowledgements
|
V |
|
1. Caenorhabditis elegans:
A Tiny Worm That's Major Model System
Introduction 1 *
Characteristics of C.elegans 1 * Procedure 1
|
1 |
|
2. Ceratopteris richardii:
A Versatile Plant Model System
Introduction 5 * The
Gametophyte Generation 6 *
The Ceratopteris Life Cycle 7 * Ceratopteris
Genetics 7 * Procedure 8
|
5 |
3. Introduction to Drosophila
and Conducting Crosses
Introduction 13 * Procedures 13
|
13 |
4. Mitosis, Meiosis, and
Observing Polytene Chromosomes in Drosophila
Introduction 23 * Procedure 23
Procedure for Preparing Drosophila
Polytene Chromosomes 31
|
23 |
5. Arabidopsis thaliana:
Searching for New Mutations
Introduction 35 * Procedure 37
|
35 |
6. Designing PCR Primers
Introduction 43
Polymerase Chain Reaction: Background
Information 43 * Procedure 44
|
43 |
7. Using PCR to Find New Gene Sequences
Introduction 49 * Procedure 49
|
49 |
8.
Mapping a Molecular Marker in Drosophlia
Introduction 57 * Strategies
for Genome Characterization 57
Background Information for the Lab
Procedure 59 * Procedure 62
|
57 |
9. Yeast as a Model System:
Conditional Mutations
Introduction 65 * The Yeast Life
Cycle 65 * Procedure 67
|
65 |
10. Isolation of Plasmid DNA
Introduction 73 *
Procedure 73
|
73 |
|
11. Genetic Transformation fo Yeast
Introduction 77 * Procedure 78
|
77 |
12. Isolation of Novel Genes from a
cDNA Library
Introduction 83 * The
Selection Scheme 83 * Procedure 88
|
83 |
13.
Using a Molecular Marker to Study Genetic Equilibrium in Drosophila
melanogaster
Introduction 93 * Procedure 95 *
Required Analysis 97 * Reference Cited 98
|
93 |
14. Pollen Tube Formation and
the Central Dogma of Biology
Introduction 99 * Procedure 101 *
Analysis 102
|
99 |
