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Title: Contemporary Genetics Laboratory Manual

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Table of Contents
Page
Preface iii
Acknowledgements V

1. Caenorhabditis elegans: A Tiny Worm That's Major Model System 
     
Introduction 1 *  Characteristics of C.elegans 1 * Procedure 1

1

2. Ceratopteris richardii: A Versatile Plant Model System
     
Introduction 5 *  The Gametophyte Generation 6 * 
      The Ceratopteris Life Cycle 7 * Ceratopteris Genetics 7 * Procedure 8

5

3. Introduction to Drosophila and Conducting Crosses
     
Introduction 13 * Procedures 13

13

4. Mitosis, Meiosis, and Observing Polytene Chromosomes in Drosophila
     
Introduction 23 * Procedure 23
      Procedure for Preparing Drosophila Polytene Chromosomes 31

23

5. Arabidopsis thaliana: Searching for New Mutations
     
Introduction 35 * Procedure 37

35

6. Designing PCR Primers
     
Introduction 43
      Polymerase Chain Reaction: Background Information 43 * Procedure 44

43
7. Using PCR to Find New Gene Sequences
     
Introduction 49 * Procedure 49
49

8. Mapping a Molecular Marker in Drosophlia
     
Introduction 57 * Strategies for Genome Characterization 57
      Background Information for the Lab Procedure 59 * Procedure 62

57

9. Yeast as a Model System: Conditional Mutations
     
Introduction 65 * The Yeast Life Cycle 65 * Procedure 67

65

10. Isolation of Plasmid DNA
     
Introduction 73 * Procedure 73

73

11. Genetic Transformation fo Yeast
     
Introduction 77 * Procedure 78

77

12. Isolation of Novel Genes from a cDNA Library
     
Introduction 83 * The Selection Scheme 83 * Procedure 88

83

13. Using a Molecular Marker to Study Genetic Equilibrium in Drosophila
      melanogaster

     
Introduction 93 * Procedure 95 * Required Analysis 97 * Reference Cited 98

93

14. Pollen Tube Formation and the Central Dogma of Biology
     
Introduction 99 * Procedure 101 * Analysis 102

99
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