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Title: Arabidopsis A Practical Approach

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Table of Contents

     2.  Preservation and handling of stock centre clones - p.29

        (Randy Scholl, Keith Davis, and Doreen Ware)

  1. Introduction

  2. Missions of a plant DNA resource centre

  3. Preservation of stocks
         Plasmids with small DNA inserts
         Cosmids
         Phage and phage libraries
         Yeast artificial chromosome (YAC) libraries
         Pools of YAC library cells for PCR screening
         Distribution of YAC libraries arrayed on nylon filters
         Other large-insert libraries
         Yeast expression analysis---'two-hybrid' libraries and complementation testing

  4. Verification of stock identify and purity

  5. Pooled DNA from T-DNA lines for PCR screening

  6. Organization of stock information
         Collecting, maintaining, and disseminating stock data
    Organizing and distributing patron data

  7. The future

     References

Page

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