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Title: Arabidopsis A Practical Approach

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Table of Contents

     3.   Genetic mapping using recombinant inbred lines

          (Clare Lister, Mary Anderson, and Caroline Dean)

  1. Introduction

  2. Preparation and digestion of A. thaliana genomic DNA
         Preparation of genomic DNA
         Identifying an RFLP
         Southern blotting and hybridization

  3. Polymorphic markers
         RFLP markers
         PCR-based markers
         Phenotypic and biochemical markers

  4. Calculating map positions
         Mapping programs
         NASC mapping service

  5. Integration of a mutation into a molecular map

     Reference

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